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Identification and evaluation of midgut protein RL12 of Dermacentor silvarum interacting with Anaplasma ovis VirD4.
- Source :
-
Ticks and tick-borne diseases [Ticks Tick Borne Dis] 2021 May; Vol. 12 (3), pp. 101677. Date of Electronic Publication: 2021 Jan 27. - Publication Year :
- 2021
-
Abstract
- Anaplasma ovis, a tick-borne intra-erythrocytic Gram-negative bacterium, is a causative agent of ovine anaplasmosis. It is known that Dermacentor ticks act as biological vectors for A. ovis. VirD4 is the machine component of Type IV Secretion System of A. ovis. To better understand the pathogen-vector interaction, VirD4 was used as a bait protein for screening midgut proteins of Dermacentor silvarum via yeast two-hybrid mating assay. As a result, a ribosomal protein RL12 was identified from the midgut cDNA library of D. silvarum. For further validation, using in vitro Glutathione S-transferase (GST) pull-down assay, interaction between the proteins, GST-RL12 and HIS-VirD4, was observed in Western blot analysis. The study is first of its kind reporting a D. silvarum midgut protein interaction with VirD4 from A. ovis. Functional annotations showed some important cellular processes are attributed to the protein, particularly in the stringent response and biogenesis. The results of the study suggest the involvement of the VirD4-RL12 interaction in the regulation of signaling pathways, which is a tool for understanding the pathogen-vector interaction.<br /> (Copyright © 2021 Elsevier GmbH. All rights reserved.)
- Subjects :
- Anaplasma ovis metabolism
Animals
Arachnid Vectors metabolism
Arachnid Vectors microbiology
Arthropod Proteins metabolism
Bacterial Proteins metabolism
Dermacentor metabolism
Dermacentor microbiology
Digestive System metabolism
Digestive System microbiology
Ribosomal Proteins metabolism
Anaplasma ovis genetics
Arachnid Vectors genetics
Arthropod Proteins genetics
Bacterial Proteins genetics
Dermacentor genetics
Ribosomal Proteins genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1877-9603
- Volume :
- 12
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Ticks and tick-borne diseases
- Publication Type :
- Academic Journal
- Accession number :
- 33549977
- Full Text :
- https://doi.org/10.1016/j.ttbdis.2021.101677