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Response of transgenic Arabidopsis expressing maize C 4 photosynthetic enzyme genes to high light.
- Source :
-
Plant signaling & behavior [Plant Signal Behav] 2021 Apr 03; Vol. 16 (4), pp. 1885894. Date of Electronic Publication: 2021 Feb 10. - Publication Year :
- 2021
-
Abstract
- This study assessed the responses of wild-type (WT) and transgenic Arabidopsis expressing seven combinations of maize ( Zea mays ) genes phosphoenolpyruvate carboxylase ( pepc ), pyruvate phosphate dikinase ( ppdk ), and NADP-malic enzyme ( nadp-me ) to high light. Our results showed that the net CO <subscript>2</subscript> assimilation rate ( P <subscript>n</subscript> ) and shoot dry weight of four of the transgenic Arabidopsis genotypes were significantly different from those of WT under high-light treatment, being in the order of Zmpepc+Zmppdk+Zmnadp-me (PC-K-M) > Zmpepc+Zmppdk (PC-K) > Zmpepc (PC), Zmpepc+Zmnadp-me (PC-M) > WT. The other genotypes did not differ from WT. This indicated that Zmpepc was essential for maintaining high photosynthetic performance under high light, Zmppdk had a positive synergistic effect on Zmpepc , and the combination of all three genes had the greatest synergistic effect. These four genotypes also maintained higher photosystem II (PSII) activity (K-phase, J-phase, RC/CSm), electron transfer capacity (J-phase), and photochemical efficiency (TRo/ABS), and accumulated less reactive oxygen species (O <subscript>2</subscript> · <superscript>-</superscript> , H <subscript>2</subscript> O <subscript>2</subscript> ) and suffered less damage to the membrane system (MDA) than WT under high light. Collectively, PC, PC-K, PC-M, and PC-K-M used most of the absorbed energy for CO <subscript>2</subscript> assimilation through a significantly higher P <subscript>n</subscript> , which reduced the generation of excess electrons in the photosynthetic apparatus, thereby reducing damage to the membrane system and PSII. This ultimately resulted in improved high-light tolerance. P <subscript>n</subscript> was the main reason for the significant difference in the high-light tolerance of the four genotypes. Joint expression of the three maize genes may be of great value in the genetic improvement of high-light tolerance in C <subscript>3</subscript> crops.
- Subjects :
- Biomass
Carbon Dioxide metabolism
Chlorophyll metabolism
Fluorescence
Hydrogen Peroxide metabolism
Malondialdehyde metabolism
Photosystem II Protein Complex metabolism
Plant Shoots growth & development
Plant Shoots radiation effects
Plants, Genetically Modified
Superoxides metabolism
Arabidopsis genetics
Arabidopsis radiation effects
Genes, Plant
Light
Photosynthesis genetics
Photosynthesis radiation effects
Zea mays enzymology
Zea mays genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1559-2324
- Volume :
- 16
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Plant signaling & behavior
- Publication Type :
- Academic Journal
- Accession number :
- 33566717
- Full Text :
- https://doi.org/10.1080/15592324.2021.1885894