Back to Search
Start Over
Molecular characterization of carbapenem-resistant Enterobacteriaceae and emergence of tigecycline non-susceptible strains in the Henan province in China: a multicentrer study.
- Source :
-
Journal of medical microbiology [J Med Microbiol] 2021 Mar; Vol. 70 (3). Date of Electronic Publication: 2021 Feb 15. - Publication Year :
- 2021
-
Abstract
- Introduction. Carbapenem-resistant Enterobacteriaceae (CRE) have been responsible for nosocomial outbreaks worldwide and have become endemic in several countries. Hypothesis/Gap Statement. To better understand the epidemiological trends and characteristics of CRE in the Henan province. Aim. We assessed the molecular epidemiological characteristics of 305 CRE strains isolated from patients in 19 secondary or tertiary hospitals in ten areas of the Henan province in China. Methodology. A total of 305 CRE isolates were subjected to multiple tests, including in vitro antimicrobial susceptibility testing, PCR for carbapenemase genes bla <subscript>KPC</subscript> , bla <subscript>NDM</subscript> , bla <subscript>IMP</subscript> , bla <subscript>VIM</subscript> , bla <subscript>OXA</subscript> - <subscript>48-like</subscript> . Tigecycline-resistant genes ramR , oqxR , acrR , tet A, rpsJ , tet X, tet M, tet L were analysed in five tigecycline non-susceptible carbapenem-resistant Klebsiella pneumoniae isolates (TNSCRKP). Additionally, multilocus sequence typing (MLST) was performed for carbapenem-resistant K. pneumoniae (CRKP). Results. The most common CRE species were K. pneumoniae (234, 77 %), Escherichia coli (36, 12 %) and Enterobacter cloacae (13, 4 %). All strains exhibited multi-drug resistance. Overall, 97 % (295/305) and 97 % (297/305) of the isolates were susceptible to polymyxin B and tigecycline, respectively. A total of 89 % (271/305) of the CRE isolates were carbapenemase gene-positive, including 70 % bla <subscript>KPC</subscript> , 13 % bla <subscript>NDM</subscript> , 6 % bla <subscript>IMP</subscript> , and 1 % combined bla <subscript>KPC</subscript> / bla <subscript>NDM</subscript> genes. K. pneumoniae carbapenemase (KPC) was the predominant carbapenemase in K. pneumoniae (87 %), whereas NDM and IMP were frequent in E. coli (53 %) and E. cloacae (69 %), respectively. Mutations in the ramR , tetA, and rpsJ genes were detected in five TNSCRKP. Moreover, 15 unique sequence types were detected, with ST11 (74 %), ST15 (9 %) and ST2237 (5 %) being dominant among K. pneumoniae strains. Conclusion. A high proportion of CRE strains were carbapenemase-positive, and five carbapenem-resistant K. pneumonia isolates were tigecycline non-susceptible, indicating a need for the ongoing surveillance of CRE and effective measures for the prevention of CRE infections.
- Subjects :
- Bacterial Proteins genetics
Carbapenem-Resistant Enterobacteriaceae classification
Carbapenem-Resistant Enterobacteriaceae isolation & purification
China epidemiology
Drug Resistance, Multiple, Bacterial drug effects
Drug Resistance, Multiple, Bacterial genetics
Enterobacteriaceae Infections epidemiology
Genotype
Humans
Microbial Sensitivity Tests
Mutation
beta-Lactamases genetics
Anti-Bacterial Agents pharmacology
Carbapenem-Resistant Enterobacteriaceae drug effects
Carbapenem-Resistant Enterobacteriaceae genetics
Enterobacteriaceae Infections microbiology
Tigecycline pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1473-5644
- Volume :
- 70
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of medical microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 33587030
- Full Text :
- https://doi.org/10.1099/jmm.0.001325