Back to Search Start Over

From local resynchronization to global pattern recovery in the zebrafish segmentation clock.

Authors :
Uriu K
Liao BK
Oates AC
Morelli LG
Source :
ELife [Elife] 2021 Feb 15; Vol. 10. Date of Electronic Publication: 2021 Feb 15.
Publication Year :
2021

Abstract

Integrity of rhythmic spatial gene expression patterns in the vertebrate segmentation clock requires local synchronization between neighboring cells by Delta-Notch signaling and its inhibition causes defective segment boundaries. Whether deformation of the oscillating tissue complements local synchronization during patterning and segment formation is not understood. We combine theory and experiment to investigate this question in the zebrafish segmentation clock. We remove a Notch inhibitor, allowing resynchronization, and analyze embryonic segment recovery. We observe unexpected intermingling of normal and defective segments, and capture this with a new model combining coupled oscillators and tissue mechanics. Intermingled segments are explained in the theory by advection of persistent phase vortices of oscillators. Experimentally observed changes in recovery patterns are predicted in the theory by temporal changes in tissue length and cell advection pattern. Thus, segmental pattern recovery occurs at two length and time scales: rapid local synchronization between neighboring cells, and the slower transport of the resulting patterns across the tissue through morphogenesis.<br />Competing Interests: KU, BL, AO, LM No competing interests declared<br /> (© 2021, Uriu et al.)

Details

Language :
English
ISSN :
2050-084X
Volume :
10
Database :
MEDLINE
Journal :
ELife
Publication Type :
Academic Journal
Accession number :
33587039
Full Text :
https://doi.org/10.7554/eLife.61358