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Elevated blood purine levels as a biomarker of seizures and epilepsy.
- Source :
-
Epilepsia [Epilepsia] 2021 Mar; Vol. 62 (3), pp. 817-828. Date of Electronic Publication: 2021 Feb 18. - Publication Year :
- 2021
-
Abstract
- Objective: There is a major unmet need for a molecular biomarker of seizures or epilepsy that lends itself to fast, affordable detection in an easy-to-use point-of-care device. Purines such as adenosine triphosphate and adenosine are potent neuromodulators released during excessive neuronal activity that are also present in biofluids. Their biomarker potential for seizures and epilepsy in peripheral blood has, however, not yet been investigated. The aim of the present study was to determine whether blood purine nucleoside measurements can serve as a biomarker for the recent occurrence of seizures and to support the diagnosis of epilepsy.<br />Methods: Blood purine concentrations were measured via a point-of-care diagnostic technology based on the summated electrochemical detection of adenosine and adenosine breakdown products (inosine, hypoxanthine, and xanthine; SMARTChip). Measurements of blood purine concentrations were carried out using samples from mice subjected to intra-amygdala kainic acid-induced status epilepticus and in video-electroencephalogram (EEG)-monitored adult patients with epilepsy.<br />Results: In mice, blood purine concentrations were rapidly increased approximately two- to threefold after status epilepticus (2.32 ± .40 µmol·L <superscript>-1</superscript> [control] vs. 8.93 ± 1.03 µmol·L <superscript>-1</superscript> [after status epilepticus]), and levels correlated with seizure burden and postseizure neurodegeneration in the hippocampus. Blood purine concentrations were also elevated in patients with video-EEG-diagnosed epilepsy (2.39 ± .34 µmol·L <superscript>-1</superscript> [control, n = 13] vs. 4.35 ± .38 µmol·L <superscript>-1</superscript> [epilepsy, n = 26]).<br />Significance: Our data provide proof of concept that the measurement of blood purine concentrations may offer a rapid, low-volume bedside test to support the diagnosis of seizures and epilepsy.<br /> (© 2021 International League Against Epilepsy.)
- Subjects :
- Adenosine blood
Adult
Animals
Biomarkers blood
Case-Control Studies
Epilepsy diagnosis
Humans
Hypoxanthine blood
Inosine blood
Male
Mice
Mice, Inbred C57BL
Middle Aged
Point-of-Care Testing
Seizures diagnosis
Severity of Illness Index
Status Epilepticus blood
Status Epilepticus diagnosis
Xanthine blood
Young Adult
Epilepsy blood
Purines blood
Seizures blood
Subjects
Details
- Language :
- English
- ISSN :
- 1528-1167
- Volume :
- 62
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Epilepsia
- Publication Type :
- Academic Journal
- Accession number :
- 33599287
- Full Text :
- https://doi.org/10.1111/epi.16839