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Exploring beyond clinical routine SARS-CoV-2 serology using MultiCoV-Ab to evaluate endemic coronavirus cross-reactivity.
- Source :
-
Nature communications [Nat Commun] 2021 Feb 19; Vol. 12 (1), pp. 1152. Date of Electronic Publication: 2021 Feb 19. - Publication Year :
- 2021
-
Abstract
- The humoral immune response to SARS-CoV-2 is a benchmark for immunity and detailed analysis is required to understand the manifestation and progression of COVID-19, monitor seroconversion within the general population, and support vaccine development. The majority of currently available commercial serological assays only quantify the SARS-CoV-2 antibody response against individual antigens, limiting our understanding of the immune response. To overcome this, we have developed a multiplex immunoassay (MultiCoV-Ab) including spike and nucleocapsid proteins of SARS-CoV-2 and the endemic human coronaviruses. Compared to three broadly used commercial in vitro diagnostic tests, our MultiCoV-Ab achieves a higher sensitivity and specificity when analyzing a well-characterized sample set of SARS-CoV-2 infected and uninfected individuals. We find a high response against endemic coronaviruses in our sample set, but no consistent cross-reactive IgG response patterns against SARS-CoV-2. Here we show a robust, high-content-enabled, antigen-saving multiplex assay suited to both monitoring vaccination studies and facilitating epidemiologic screenings for humoral immunity towards pandemic and endemic coronaviruses.
- Subjects :
- COVID-19 diagnosis
Coronavirus Nucleocapsid Proteins immunology
Humans
Immunoassay
Immunoglobulin G immunology
Phosphoproteins immunology
SARS-CoV-2 immunology
Sensitivity and Specificity
Spike Glycoprotein, Coronavirus immunology
Antibodies, Viral immunology
COVID-19 immunology
COVID-19 Serological Testing methods
Cross Reactions
Immunity, Humoral
Subjects
Details
- Language :
- English
- ISSN :
- 2041-1723
- Volume :
- 12
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Nature communications
- Publication Type :
- Academic Journal
- Accession number :
- 33608538
- Full Text :
- https://doi.org/10.1038/s41467-021-20973-3