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Phenotypic technologies in stem cell biology.
- Source :
-
Cell chemical biology [Cell Chem Biol] 2021 Mar 18; Vol. 28 (3), pp. 257-270. Date of Electronic Publication: 2021 Mar 01. - Publication Year :
- 2021
-
Abstract
- The high-throughput phenotypic screen (HTPS) has become an emerging technology to discover synthetic small molecules that regulate stem cell fates. Here, we review the application of HTPS to identify small molecules controlling stem cell renewal, reprogramming, differentiation, and lineage conversion. Moreover, we discuss the use of HTPS to discover small molecules/polymers mimicking the stem cell extracellular niche. Furthermore, HTPSs have been applied on whole-animal models to identify small molecules regulating stem cell renewal or differentiation in vivo. Finally, we discuss the examples of the utilization of HTPS in stem cell-based disease modeling, as well as in the discovery of novel drug candidates for cancer, diabetes, and infectious diseases. Overall, HTPSs have provided many powerful tools for the stem cell field, which not only facilitate the generation of functional cells/tissues for replacement therapy, disease modeling, and drug screening, but also help dissect molecular mechanisms regulating physiological and pathological processes.<br />Competing Interests: Declaration of interests The authors declare no competing interests.<br /> (Copyright © 2021 Elsevier Ltd. All rights reserved.)
Details
- Language :
- English
- ISSN :
- 2451-9448
- Volume :
- 28
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Cell chemical biology
- Publication Type :
- Academic Journal
- Accession number :
- 33651977
- Full Text :
- https://doi.org/10.1016/j.chembiol.2021.02.001