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Tamlana haliotis sp. nov., isolated from the gut of the abalone Haliotis rubra.
- Source :
-
Archives of microbiology [Arch Microbiol] 2021 Jul; Vol. 203 (5), pp. 2357-2364. Date of Electronic Publication: 2021 Mar 03. - Publication Year :
- 2021
-
Abstract
- A Gram-stain-negative, aerobic, non-motile, yellow-pigmented rod-shaped and alginate-degrading bacterium, designated B1N29 <superscript>T</superscript> , was isolated from the gut of the abalone Haliotis rubra obtained in Weihai, China. Strain B1N29 <superscript>T</superscript> was found to grow at 4-35 ℃ (optimum, 25 ℃), at pH 6.5-9.0 (optimum, 7.0-7.5) and in the presence of 0.5-9% (w/v) NaCl (optimum, 2%). Cells were positive for oxidase and catalase activity. The 16S rRNA-based phylogenetic analysis revealed that the nearest phylogenetic neighbors of strain B1N29 <superscript>T</superscript> were Tamlana carrageenivorans KCTC 62451 <superscript>T</superscript> (98.2%) and Tamlana agarivorans KCTC 22176 <superscript>T</superscript> (97.7%). Based on the phylogenomic analysis, the average nucleotide identity (ANI) values between strain B1N29 <superscript>T</superscript> and the neighbor strains were 79.2 and 79.0%, respectively; the digital DNA-DNA hybridization (dDDH) values between strain B1N29 <superscript>T</superscript> and its two closest neighbors were 22.8 and 23.0%, respectively. Menaquinone-6 (MK-6) was detected as the sole respiratory quinone. The dominant cellular fatty acids were iso-C <subscript>15:0</subscript> , iso-C <subscript>17:0</subscript> 3-OH, anteiso-C <subscript>15:0</subscript> and iso-C <subscript>15:1</subscript> G. The polar lipids included phosphatidylethanolamine, one aminophospholipid, seven aminolipids and five unidentified lipids. Based on the phylogenetic and phenotypic characteristics, strain B1N29 <superscript>T</superscript> is considered to represent a novel species of the genus Tamlana, for which the name Tamlana haliotis sp. nov. is proposed. The type strain is B1N29 <superscript>T</superscript> (= KCTC 72683 <superscript>T</superscript> = MCCC 1H00394 <superscript>T</superscript> ).
- Subjects :
- Animals
Bacterial Typing Techniques
Base Composition
China
DNA, Bacterial genetics
Fatty Acids chemistry
Flavobacteriaceae isolation & purification
Nucleic Acid Hybridization
Phospholipids chemistry
Pigmentation
RNA, Ribosomal, 16S genetics
Sequence Analysis, DNA
Vitamin K 2 analogs & derivatives
Vitamin K 2 chemistry
Flavobacteriaceae classification
Gastrointestinal Tract microbiology
Gastropoda microbiology
Phylogeny
Subjects
Details
- Language :
- English
- ISSN :
- 1432-072X
- Volume :
- 203
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Archives of microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 33656587
- Full Text :
- https://doi.org/10.1007/s00203-021-02216-7