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Antioxidant and Antisteatotic Activities of a New Fucoidan Extracted from Ferula hermonis Roots Harvested on Lebanese Mountains.
- Source :
-
Molecules (Basel, Switzerland) [Molecules] 2021 Feb 22; Vol. 26 (4). Date of Electronic Publication: 2021 Feb 22. - Publication Year :
- 2021
-
Abstract
- Fucoidan is a fucose-rich sulfated polysaccharide with attractive therapeutic potential due to a variety of biological activities, including antioxidant action. Fucoidan is typically found in the cell wall of marine brown algae, but extra-algal sources have also been discovered. In the present work, for the first time we extracted a water soluble fucoidan fraction from the roots of the terrestrial shrub Ferula hermonis. This fucoidan fraction was termed FUFe, and contained fucose, glucose, sulfate, smaller amounts of monosaccharides such as galactose and mannose, and a minor quantity of proteins. FUFe structural features were investigated by FTIR, <superscript>1</superscript> H NMR and <superscript>13</superscript> C NMR spectroscopy. The antioxidant property of FUFe was measured by DPPH, ABTS and FRAP assays, which revealed a high radical scavenging capacity that was confirmed in in vitro cellular models. In hepatic and endothelial cells, 50 μg/mL FUFe could reduce ROS production induced by intracellular lipid accumulation. Moreover, in hepatic cells FUFe exhibited a significant antisteatotic action, being able to reduce intracellular triglyceride content and to regulate the expression of key genes of hepatic lipid metabolism. Altogether, our results candidate FUFe as a possible bioactive compound against fatty liver disease and related vascular damage.
- Subjects :
- Animals
Antioxidants chemistry
Antioxidants isolation & purification
Benzothiazoles antagonists & inhibitors
Biphenyl Compounds antagonists & inhibitors
Cells, Cultured
Fluorescence Recovery After Photobleaching
Humans
Lebanon
Picrates antagonists & inhibitors
Plant Extracts chemistry
Plant Extracts isolation & purification
Polysaccharides chemistry
Polysaccharides isolation & purification
Rats
Reactive Oxygen Species metabolism
Sulfonic Acids antagonists & inhibitors
Antioxidants pharmacology
Ferula chemistry
Plant Extracts pharmacology
Plant Roots chemistry
Polysaccharides pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1420-3049
- Volume :
- 26
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Molecules (Basel, Switzerland)
- Publication Type :
- Academic Journal
- Accession number :
- 33671715
- Full Text :
- https://doi.org/10.3390/molecules26041161