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Preparation of Active Chitooligosaccharides with a Novel Chitosanase Aq CoA and Their Application in Fungal Disease Protection.
- Source :
-
Journal of agricultural and food chemistry [J Agric Food Chem] 2021 Mar 24; Vol. 69 (11), pp. 3351-3361. Date of Electronic Publication: 2021 Mar 10. - Publication Year :
- 2021
-
Abstract
- Enzymes that degrade fungal cell walls and the resulting oligosaccharides are promising weapons to combat plant fungal disease. In this study, we identified a novel endo-chitosanase, Aq CoA, from Aquabacterium sp. A7-Y. The enzyme showed a specific activity of 18 U/mg toward 95% deacetylated chitosan at pH 5.0 and 40 °C. Aq CoA also showed activity toward sodium carboxymethylcellulose, indicating substrate promiscuity. Aq CoA hydrolyzed chitosan into chitooligosaccharides (CoA-COSs) with degrees of polymerization (DPs) of 3-5 but showed no activity toward CoA-COSs with DPs <6, indicating an endo-type activity. At 2.5 μg/mL, Aq CoA inhibited appressorium formation of Magnaporthe oryzae ; the produced CoA-COSs also inhibited the growth of M. oryzae and Fusarium oxysporum . Furthermore, CoA-COSs acted as immune elicitors in rice by inducing the reactive oxygen species burst and the expression of defense genes. These results demonstrated that Aq CoA and its resulting CoA-COSs might be effective tools for protecting plants against pathogenic fungi.
Details
- Language :
- English
- ISSN :
- 1520-5118
- Volume :
- 69
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Journal of agricultural and food chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 33688732
- Full Text :
- https://doi.org/10.1021/acs.jafc.0c07802