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Ralstonia solanacearum type III effector RipV2 encoding a novel E3 ubiquitin ligase (NEL) is required for full virulence by suppressing plant PAMP-triggered immunity.
- Source :
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Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2021 Apr 23; Vol. 550, pp. 120-126. Date of Electronic Publication: 2021 Mar 07. - Publication Year :
- 2021
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Abstract
- Ralstonia solanacearum causes bacterial wilt disease in a broad range of plants, primarily through type Ⅲ secreted effectors. However, the R. solanacearum effectors promoting susceptibility in host plants remain limited. In this study, we determined that the R. solanacearum effector RipV2 functions as a novel E3 ubiquitin ligase (NEL). RipV2 was observed to be locali in the plasma membrane after translocatio into plant cells. Transient expression of RipV2 in Nicotiana benthamiana could induce cell death and suppress the flg22-induced pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) responses, mediating such effects as attenuation of the expression of several PTI-related genes and ROS bursts. Furthermore, we demonstrated that the conserved catalytic residue is highly important for RipV2. Transient expression of the E3 ubiquitin ligase catalytic mutant RipV2 C403A alleviated the PTI suppression ability and cell death induction, indicating that RipV2 requires its E3 ubiquitin ligase activity for its role in plant-microbe interactions. More importantly, mutation of RipV2 in R. solanacearum reduces the virulence of R. solanacearum on potato. In conclusion, we identified a NEL effector that is required for full virulence of R. solanacearum by suppressing plant PTI.<br />Competing Interests: Declaration of competing interest The authors have declared no conflicts of interest.<br /> (Copyright © 2021 Elsevier Inc. All rights reserved.)
- Subjects :
- Amino Acid Motifs
Biocatalysis
Cell Death
Cell Membrane enzymology
Cysteine metabolism
Flagellin chemistry
Flagellin immunology
Pathogen-Associated Molecular Pattern Molecules immunology
Peptide Fragments chemistry
Peptide Fragments immunology
Ralstonia solanacearum genetics
Ubiquitin-Protein Ligases chemistry
Pathogen-Associated Molecular Pattern Molecules antagonists & inhibitors
Plant Immunity
Ralstonia solanacearum enzymology
Solanum tuberosum immunology
Solanum tuberosum microbiology
Ubiquitin-Protein Ligases genetics
Ubiquitin-Protein Ligases metabolism
Virulence genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1090-2104
- Volume :
- 550
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 33691198
- Full Text :
- https://doi.org/10.1016/j.bbrc.2021.02.082