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Reducing NF-κB Signaling Nutritionally is Associated with Expedited Recovery of Skeletal Muscle Function After Damage.
- Source :
-
The Journal of clinical endocrinology and metabolism [J Clin Endocrinol Metab] 2021 Jun 16; Vol. 106 (7), pp. 2057-2076. - Publication Year :
- 2021
-
Abstract
- Context: The early events regulating the remodeling program following skeletal muscle damage are poorly understood.<br />Objective: The objective of this study was to determine the association between myofibrillar protein synthesis (myoPS) and nuclear factor-kappa B (NF-κB) signaling by nutritionally accelerating the recovery of muscle function following damage.<br />Design, Setting, Participants, and Interventions: Healthy males and females consumed daily postexercise and prebed protein-polyphenol (PP; n = 9; 4 females) or isocaloric maltodextrin placebo (PLA; n = 9; 3 females) drinks (parallel design) 6 days before and 3 days after 300 unilateral eccentric contractions of the quadriceps during complete dietary control.<br />Main Outcome Measures: Muscle function was assessed daily, and skeletal muscle biopsies were taken after 24, 27, and 36 hours for measurements of myoPS rates using deuterated water, and gene ontology and NF-κB signaling analysis using a quantitative reverse transcription PCR (RT-qPCR) gene array.<br />Results: Eccentric contractions impaired muscle function for 48 hours in PLA intervention, but just for 24 hours in PP intervention (P = 0.047). Eccentric quadricep contractions increased myoPS compared with the control leg during postexercise (24-27 hours; 0.14 ± 0.01 vs 0.11 ± 0.01%·h-1, respectively; P = 0.075) and overnight periods (27-36 hours; 0.10 ± 0.01 vs 0.07 ± 0.01%·h-1, respectively; P = 0.020), but was not further increased by PP drinks (P > 0.05). Protein-polyphenol drinks decreased postexercise and overnight muscle IL1R1 (PLA = 2.8 ± 0.4, PP = 1.1 ± 0.4 and PLA = 1.9 ± 0.4, PP = 0.3 ± 0.4 log2 fold-change, respectively) and IL1RL1 (PLA = 4.9 ± 0.7, PP = 1.6 ± 0.8 and PLA = 3.7 ± 0.6, PP = 0.7 ± 0.7 log2 fold-change, respectively) messenger RNA expression (P < 0.05) and downstream NF-κB signaling compared with PLA.<br />Conclusion: Protein-polyphenol drink ingestion likely accelerates recovery of muscle function by attenuating inflammatory NF-κB transcriptional signaling, possibly to reduce aberrant tissue degradation rather than increase myoPS rates.<br /> (© The Author(s) 2021. Published by Oxford University Press on behalf of the Endocrine Society.)
- Subjects :
- Dietary Proteins administration & dosage
Female
Healthy Volunteers
Humans
Male
Muscle Contraction drug effects
Muscle Proteins drug effects
Muscle, Skeletal physiopathology
Myalgia physiopathology
NF-kappa B metabolism
Polyphenols administration & dosage
Protein Biosynthesis drug effects
Quadriceps Muscle physiopathology
Resistance Training adverse effects
Young Adult
Beverages
Myalgia diet therapy
Recovery of Function drug effects
Signal Transduction drug effects
Sports Nutritional Physiological Phenomena drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1945-7197
- Volume :
- 106
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- The Journal of clinical endocrinology and metabolism
- Publication Type :
- Academic Journal
- Accession number :
- 33710344
- Full Text :
- https://doi.org/10.1210/clinem/dgab106