Investigation of the Ferredoxin's Influence on the Anaerobic and Aerobic, Enzymatic H 2 Production.

Ferredoxins are metalloproteins that deliver electrons to several redox partners, including [FeFe] hydrogenases that are potentially a component of biological H ₂ production technologies. Reduced ferredoxins can also lose electrons to molecular oxygen, which may lower the availability of electrons for cellular or synthetic reactions. Ferredoxins thus play a key role in diverse kinds of redox biochemistry, especially the enzymatic H ₂ production catalyzed by [FeFe] hydrogenases. We investigated how the yield of anaerobic and aerobic H ₂ production vary among the four different types of ferredoxins that are used to deliver electrons extracted from NADPH within the synthetic, fermentative pathway. We also assessed the electron loss due to O ₂ reduction by reduced ferredoxins within the pathway, for which the difference was as high as five-fold. Our findings provide valuable insights for further improving biological H ₂ production technologies and can also facilitate elucidation of mechanisms governing interactions between Fe-S cluster(s) and molecular oxygen.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The handling editor declared a past co-authorship with one of the authors, JK.
(Copyright © 2021 Koo and Cha.)