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Memory B cell repertoire for recognition of evolving SARS-CoV-2 spike.

Authors :
Tong P
Gautam A
Windsor I
Travers M
Chen Y
Garcia N
Whiteman NB
McKay LGA
Lelis FJN
Habibi S
Cai Y
Rennick LJ
Duprex WP
McCarthy KR
Lavine CL
Zuo T
Lin J
Zuiani A
Feldman J
MacDonald EA
Hauser BM
Griffths A
Seaman MS
Schmidt AG
Chen B
Neuberg D
Bajic G
Harrison SC
Wesemann DR
Source :
BioRxiv : the preprint server for biology [bioRxiv] 2021 Mar 10. Date of Electronic Publication: 2021 Mar 10.
Publication Year :
2021

Abstract

Memory B cell reserves can generate protective antibodies against repeated SARS-CoV-2 infections, but with an unknown reach from original infection to antigenically drifted variants. We charted memory B cell receptor-encoded monoclonal antibodies (mAbs) from 19 COVID-19 convalescent subjects against SARS-CoV-2 spike (S) and found 7 major mAb competition groups against epitopes recurrently targeted across individuals. Inclusion of published and newly determined structures of mAb-S complexes identified corresponding epitopic regions. Group assignment correlated with cross-CoV-reactivity breadth, neutralization potency, and convergent antibody signatures. mAbs that competed for binding the original S isolate bound differentially to S variants, suggesting the protective importance of otherwise-redundant recognition. The results furnish a global atlas of the S-specific memory B cell repertoire and illustrate properties conferring robustness against emerging SARS-CoV-2 variants.<br />Competing Interests: Competing interests The authors declare no competing interests.

Details

Language :
English
ISSN :
2692-8205
Database :
MEDLINE
Journal :
BioRxiv : the preprint server for biology
Accession number :
33758863
Full Text :
https://doi.org/10.1101/2021.03.10.434840