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The Burkholderia pseudomallei intracellular 'TRANSITome'.
- Source :
-
Nature communications [Nat Commun] 2021 Mar 26; Vol. 12 (1), pp. 1907. Date of Electronic Publication: 2021 Mar 26. - Publication Year :
- 2021
-
Abstract
- Prokaryotic cell transcriptomics has been limited to mixed or sub-population dynamics and individual cells within heterogeneous populations, which has hampered further understanding of spatiotemporal and stage-specific processes of prokaryotic cells within complex environments. Here we develop a 'TRANSITomic' approach to profile transcriptomes of single Burkholderia pseudomallei cells as they transit through host cell infection at defined stages, yielding pathophysiological insights. We find that B. pseudomallei transits through host cells during infection in three observable stages: vacuole entry; cytoplasmic escape and replication; and membrane protrusion, promoting cell-to-cell spread. The B. pseudomallei 'TRANSITome' reveals dynamic gene-expression flux during transit in host cells and identifies genes that are required for pathogenesis. We find several hypothetical proteins and assign them to virulence mechanisms, including attachment, cytoskeletal modulation, and autophagy evasion. The B. pseudomallei 'TRANSITome' provides prokaryotic single-cell transcriptomics information enabling high-resolution understanding of host-pathogen interactions.
- Subjects :
- Animals
Burkholderia pseudomallei cytology
Burkholderia pseudomallei pathogenicity
Cell Line, Tumor
Cell Membrane microbiology
Cytoplasm microbiology
HEK293 Cells
Host-Pathogen Interactions
Humans
Melioidosis microbiology
Mice
Mice, Inbred BALB C
RAW 264.7 Cells
Single-Cell Analysis methods
Vacuoles microbiology
Virulence genetics
Bacterial Proteins genetics
Burkholderia pseudomallei genetics
Computational Biology methods
Gene Expression Profiling methods
Genes, Bacterial genetics
Virulence Factors genetics
Subjects
Details
- Language :
- English
- ISSN :
- 2041-1723
- Volume :
- 12
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Nature communications
- Publication Type :
- Academic Journal
- Accession number :
- 33772012
- Full Text :
- https://doi.org/10.1038/s41467-021-22169-1