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Molecular characterization of the COPT/Ctr-type copper transporter family under heavy metal stress in alfalfa.
- Source :
-
International journal of biological macromolecules [Int J Biol Macromol] 2021 Jun 30; Vol. 181, pp. 644-652. Date of Electronic Publication: 2021 Mar 30. - Publication Year :
- 2021
-
Abstract
- In nature, heavy metals significantly affect crop growth and quality. Among various heavy metals, copper (Cu) is both essential and toxic to plants depending on the concentration and complex homeostatic networks. The Cu transporter family (COPT) plays important roles in Cu homeostasis, including absorption, transportation, and growth in plants; however, this gene family is still poorly understood in alfalfa (Medicago sativa L.). In this study, a total of 12 MsCOPTs were identified and characterized. Based on the conserved motif and phylogenetic analysis, MsCOPTs could be divided into four subgroups (A1, A2, A3, and B). Gene structure, chromosomal location, and synteny analyses of MsCOPTs showed that segmental and tandem duplications likely contributed to their evolution. Tissue-specific expression analysis of MsCOPT genes indicated diverse spatiotemporal expression patterns. Most MsCOPT genes had high transcription levels in roots and nodules, indicating that these genes may play vital roles in the absorption and transport of Cu through root. The complementary heterologous expression function of yeast once again indicates that root-specific COPT can supplement the growth of defective yeast strains on YPEG medium, suggesting that these genes are Cu transporters. In summary, for the first time, our research identified COPT family genes at the whole-genome level to provide guidance for effectively improving the problem of Cu deficiency in the grass-livestock chain and provide theoretical support for the subsequent development of grass and animal husbandry.<br />Competing Interests: Declaration of competing interest The author declares that there is no competitive or financial interest known to influence the work reported in this paper.<br /> (Copyright © 2021 Elsevier B.V. All rights reserved.)
- Subjects :
- Amino Acid Sequence
Chromosomes, Plant genetics
Copper Transport Proteins chemistry
Copper Transport Proteins metabolism
Gene Expression Regulation, Plant drug effects
Genome, Plant
Medicago sativa drug effects
Mutation genetics
Nucleotide Motifs
Organ Specificity drug effects
Organ Specificity genetics
Phylogeny
Plant Proteins metabolism
Promoter Regions, Genetic genetics
Protein Interaction Maps drug effects
Saccharomyces cerevisiae genetics
Saccharomyces cerevisiae growth & development
Stress, Physiological drug effects
Copper Transport Proteins genetics
Medicago sativa genetics
Medicago sativa physiology
Metals, Heavy toxicity
Multigene Family
Plant Proteins genetics
Stress, Physiological genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0003
- Volume :
- 181
- Database :
- MEDLINE
- Journal :
- International journal of biological macromolecules
- Publication Type :
- Academic Journal
- Accession number :
- 33798576
- Full Text :
- https://doi.org/10.1016/j.ijbiomac.2021.03.173