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RNA In Situ Hybridization for Detecting Gene Expression Patterns in the Abdomens and Wings of Drosophila Species.
- Source :
-
Methods and protocols [Methods Protoc] 2021 Mar 10; Vol. 4 (1). Date of Electronic Publication: 2021 Mar 10. - Publication Year :
- 2021
-
Abstract
- RNA in situ hybridization (ISH) is used to visualize spatio-temporal gene expression patterns with broad applications in biology and biomedicine. Here we provide a protocol for mRNA ISH in developing pupal wings and abdomens for model and non-model Drosophila species. We describe best practices in pupal staging, tissue preparation, probe design and synthesis, imaging of gene expression patterns, and image-editing techniques. This protocol has been successfully used to investigate the roles of genes underlying the evolution of novel color patterns in non-model Drosophila species.
Details
- Language :
- English
- ISSN :
- 2409-9279
- Volume :
- 4
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Methods and protocols
- Publication Type :
- Academic Journal
- Accession number :
- 33801802
- Full Text :
- https://doi.org/10.3390/mps4010020