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COPB2: A Novel Prognostic Biomarker That Affects Progression of HCC.
- Source :
-
BioMed research international [Biomed Res Int] 2021 Mar 20; Vol. 2021, pp. 6648078. Date of Electronic Publication: 2021 Mar 20 (Print Publication: 2021). - Publication Year :
- 2021
-
Abstract
- Purpose: This study is aimed at investigating the expression, underlying biological function, and clinical significance of coatomer protein complex subunit beta 2 (COPB2) in hepatocellular carcinoma (HCC).<br />Methods: HCC-related data were extracted from The Cancer Genome Atlas (TCGA) database, International Cancer Genome Consortium (ICGC) database, and Gene Expression Omnibus (GEO) database. A logistic regression module was applied to analyze the relationship between the expression of COPB2 and clinicopathologic characteristics. The Cox proportional hazard regression model and Kaplan-Meier method were used for survival analysis. Gene set enrichment analysis (GSEA) was used to annotate the underlying biological functions. Loss-of-function experiments were conducted to determine the underlying mechanisms.<br />Results: COPB2 was overexpressed in HCC, and high expression of COPB2 was significantly correlated with higher alpha fetoprotein (AFP) (odds ratio (OR) = 1.616, >20 vs. ≤20, p < 0.05), stage (OR = 1.744, III vs. I, p < 0.05), and grade (OR = 1.746, G4+G3 vs. G2+G1, p < 0.05). Kaplan-Meier survival analysis showed that HCC patients with high COPB2 expression had a worse prognosis than those with low COPB2 expression ( p < 0.0001 for TCGA cohort, p < 0.05 for ICGC cohort). The univariate Cox (hazard ratio (HR) = 1.068, p < 0.0001) and multivariate Cox (HR = 2.011, p < 0.05) regression analyses suggested that COPB2 was an independent risk factor. GSEA showed that mTOR and other tumor-related signaling pathways were differentially enriched in the high COPB2 expression phenotype. Silencing of COPB2 inhibited the proliferation, migration, and invasion abilities by suppressing epithelial-mesenchymal transition and mTOR signaling.<br />Conclusion: COPB2 is a novel prognostic biomarker and a promising therapeutic target for HCC.<br />Competing Interests: The authors report no conflicts of interest in this work.<br /> (Copyright © 2021 Jiayao Zhang et al.)
- Subjects :
- Cell Line, Tumor
Disease Progression
Disease-Free Survival
Female
Humans
Male
Survival Rate
Biomarkers, Tumor genetics
Biomarkers, Tumor metabolism
Carcinoma, Hepatocellular genetics
Carcinoma, Hepatocellular metabolism
Carcinoma, Hepatocellular mortality
Carcinoma, Hepatocellular pathology
Coatomer Protein genetics
Coatomer Protein metabolism
Liver Neoplasms genetics
Liver Neoplasms metabolism
Liver Neoplasms mortality
Liver Neoplasms pathology
Neoplasm Proteins genetics
Neoplasm Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2314-6141
- Volume :
- 2021
- Database :
- MEDLINE
- Journal :
- BioMed research international
- Publication Type :
- Academic Journal
- Accession number :
- 33824874
- Full Text :
- https://doi.org/10.1155/2021/6648078