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Evaluation of the PE Δ III-LC3-KDEL3 Chimeric Protein of Entamoeba histolytica- Lectin as a Vaccine Candidate against Amebic Liver Abscess.

Authors :
Martínez-Hernández SL
Becerra-González VM
Muñoz-Ortega MH
Loera-Muro VM
Ávila-Blanco ME
Medina-Rosales MN
Ventura-Juárez J
Source :
Journal of immunology research [J Immunol Res] 2021 Mar 20; Vol. 2021, pp. 6697900. Date of Electronic Publication: 2021 Mar 20 (Print Publication: 2021).
Publication Year :
2021

Abstract

Entamoeba histolytica is an intestinal parasite that causes dysentery and amebic liver abscess. E. histolytica has the capability to invade host tissue by union of virulence factor Gal/GalNAc lectin; this molecule induces an adherence-inhibitory antibody response as well as to protect against amebic liver abscess (ALA). The present work showed the effect of the immunization with PE Δ III-LC3-KDEL3 recombinant protein. In vitro , this candidate vaccine inhibited adherence of E. histolytica trophozoites to HepG2 cell monolayer, avoiding the cytolysis, and in a hamster model, we observed a vaccine-induced protection against the damage to tissue liver and the inhibition of uncontrolled inflammation. PE Δ III-LC3-KDEL3 reduced the expression of TNF- α , IL-1 β , and NF- κ B in all immunized groups at 4- and 7-day postinfection. The levels of IL-10, FOXP3, and IFN- γ were elevated at 7 days. The immunohistochemistry assay confirmed this result, revealing an elevated quantity of +IFN- γ cells in the liver tissue. ALA formation in hamsters immunized was minimal, and few trophozoites were identified. Hence, immunization with PE Δ III-LC3-KDEL3 herein prevented invasive amebiasis, avoided an acute proinflammatory response, and activated a protective response within a short time. Finally, this recombinant protein induced an increase of serum IgG.<br />Competing Interests: The authors declare that there is no conflict of interest regarding the publication of this paper.<br /> (Copyright © 2021 Sandra L. Martínez-Hernández et al.)

Details

Language :
English
ISSN :
2314-7156
Volume :
2021
Database :
MEDLINE
Journal :
Journal of immunology research
Publication Type :
Academic Journal
Accession number :
33824880
Full Text :
https://doi.org/10.1155/2021/6697900