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Evaluation of the PE Δ III-LC3-KDEL3 Chimeric Protein of Entamoeba histolytica- Lectin as a Vaccine Candidate against Amebic Liver Abscess.
- Source :
-
Journal of immunology research [J Immunol Res] 2021 Mar 20; Vol. 2021, pp. 6697900. Date of Electronic Publication: 2021 Mar 20 (Print Publication: 2021). - Publication Year :
- 2021
-
Abstract
- Entamoeba histolytica is an intestinal parasite that causes dysentery and amebic liver abscess. E. histolytica has the capability to invade host tissue by union of virulence factor Gal/GalNAc lectin; this molecule induces an adherence-inhibitory antibody response as well as to protect against amebic liver abscess (ALA). The present work showed the effect of the immunization with PE Δ III-LC3-KDEL3 recombinant protein. In vitro , this candidate vaccine inhibited adherence of E. histolytica trophozoites to HepG2 cell monolayer, avoiding the cytolysis, and in a hamster model, we observed a vaccine-induced protection against the damage to tissue liver and the inhibition of uncontrolled inflammation. PE Δ III-LC3-KDEL3 reduced the expression of TNF- α , IL-1 β , and NF- κ B in all immunized groups at 4- and 7-day postinfection. The levels of IL-10, FOXP3, and IFN- γ were elevated at 7 days. The immunohistochemistry assay confirmed this result, revealing an elevated quantity of +IFN- γ cells in the liver tissue. ALA formation in hamsters immunized was minimal, and few trophozoites were identified. Hence, immunization with PE Δ III-LC3-KDEL3 herein prevented invasive amebiasis, avoided an acute proinflammatory response, and activated a protective response within a short time. Finally, this recombinant protein induced an increase of serum IgG.<br />Competing Interests: The authors declare that there is no conflict of interest regarding the publication of this paper.<br /> (Copyright © 2021 Sandra L. Martínez-Hernández et al.)
- Subjects :
- Animals
Antibodies, Protozoan blood
Disease Models, Animal
Entamoeba histolytica genetics
Humans
Immunogenicity, Vaccine
Lectins genetics
Lectins immunology
Liver immunology
Liver parasitology
Liver pathology
Liver Abscess, Amebic blood
Liver Abscess, Amebic parasitology
Liver Abscess, Amebic pathology
Male
Mesocricetus
Protozoan Proteins genetics
Protozoan Proteins immunology
Protozoan Vaccines genetics
Protozoan Vaccines immunology
Recombinant Fusion Proteins genetics
Recombinant Fusion Proteins immunology
Vaccines, Synthetic administration & dosage
Vaccines, Synthetic genetics
Vaccines, Synthetic immunology
Entamoeba histolytica immunology
Liver Abscess, Amebic prevention & control
Protozoan Proteins administration & dosage
Protozoan Vaccines administration & dosage
Recombinant Fusion Proteins administration & dosage
Subjects
Details
- Language :
- English
- ISSN :
- 2314-7156
- Volume :
- 2021
- Database :
- MEDLINE
- Journal :
- Journal of immunology research
- Publication Type :
- Academic Journal
- Accession number :
- 33824880
- Full Text :
- https://doi.org/10.1155/2021/6697900