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Immobilization of recombinant Escherichia coli cells expressing glucose isomerase using modified diatomite as a carrier for effective production of high fructose corn syrup in packed bed reactor.
- Source :
-
Bioprocess and biosystems engineering [Bioprocess Biosyst Eng] 2021 Aug; Vol. 44 (8), pp. 1781-1792. Date of Electronic Publication: 2021 Apr 08. - Publication Year :
- 2021
-
Abstract
- To improve the operational stability of glucose isomerase in E. coli TEGI-W139F/V186T, the immobilized cells were prepared with modified diatomite as a carrier and 74.1% activity of free cells was recovered after immobilization. Results showed that the immobilized cells still retained 86.2% of the initial transformational activity after intermittent reused 40 cycles and the yield of D-fructose reached above 42% yield at 60 °C. Moreover, the immobilized cells were employed in the continuous production of High Fructose Corn Syrup (HFCS) in a recirculating packed bed reactor for 603 h at a constant flow rate. It showed that the immobilized cells exhibited good operational stability and the yield of D-fructose retained above 42% within 603 h. The space-time yield of high fructose corn syrup reached 3.84 kg L <superscript>-1</superscript>  day <superscript>-1</superscript> . The investigation provided an efficient immobilization method for recombinant cells expressing glucose isomerase with higher stability, and the immobilized cells are a promising biocatalyst for HFCS production.
- Subjects :
- Bacterial Proteins
Bioreactors
Cobalt chemistry
Enzymes, Immobilized
Fructose chemistry
Glucose
Hydrogen-Ion Concentration
Ions
Magnesium chemistry
Microscopy, Electron, Scanning
Temperature
Aldose-Ketose Isomerases chemistry
Diatomaceous Earth chemistry
Escherichia coli metabolism
High Fructose Corn Syrup chemistry
Recombinant Proteins chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1615-7605
- Volume :
- 44
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Bioprocess and biosystems engineering
- Publication Type :
- Academic Journal
- Accession number :
- 33830378
- Full Text :
- https://doi.org/10.1007/s00449-021-02560-4