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Clinical impact of genomic characterization of 15 patients with acute megakaryoblastic leukemia-related malignancies.

Authors :
Lalonde E
Rentas S
Wertheim G
Cao K
Surrey LF
Lin F
Zhao X
Obstfeld A
Aplenc R
Luo M
Li MM
Source :
Cold Spring Harbor molecular case studies [Cold Spring Harb Mol Case Stud] 2021 Apr 08; Vol. 7 (2). Date of Electronic Publication: 2021 Apr 08 (Print Publication: 2021).
Publication Year :
2021

Abstract

Acute megakaryoblastic leukemia (AMKL) is a rare subtype of acute myeloid leukemia but is approximately 500 times more likely to develop in children with Down syndrome (DS) through transformation of transient abnormal myelopoiesis (TAM). This study investigates the clinical significance of genomic heterogeneity of AMKL in children with and without DS and in children with TAM. Genomic evaluation of nine patients with DS-related TAM or AMKL, and six patients with non-DS AMKL, included conventional cytogenetics and a comprehensive next-generation sequencing panel for single-nucleotide variants/indels and copy-number variants in 118 genes and fusions involving 110 genes. Recurrent gene fusions were found in all patients with non-DS, including two individuals with complex genomes and either a NUP98-KDM5A or a KMT2A - MLLT6 fusion, and the remaining harbored a CBFA2T3-GLIS2 fusion, which arose from both typical and atypical cytogenetic mechanisms. These fusions guided treatment protocols and resulted in a change in diagnosis in two patients. The nine patients with DS had constitutional trisomy 21 and somatic GATA1 mutations, and those with DS-AMKL had two to four additional clinically significant somatic mutations. Comprehensive genomic characterization provides critical information for diagnosis, risk stratification, and treatment decisions for patients with AMKL. Continued genetic and clinical characterization of these rare cancers will aid in improving patient management.<br /> (© 2021 Lalonde et al.; Published by Cold Spring Harbor Laboratory Press.)

Details

Language :
English
ISSN :
2373-2873
Volume :
7
Issue :
2
Database :
MEDLINE
Journal :
Cold Spring Harbor molecular case studies
Publication Type :
Academic Journal
Accession number :
33832921
Full Text :
https://doi.org/10.1101/mcs.a005975