Arabidopsis thaliana Mature Endosperm Dissection and Isolation of Genomic DNA from Mature Seed Tissues.

We describe methods to separate endosperms and embryos from Arabidopsis thaliana mature seeds in large amounts and to isolate high-quality genomic DNA from those tissues. The resulting materials are suitable for analysis of DNA methylation by bisulfite sequencing or histone modifications by chromatin immunoprecipitation (ChIP).