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Dendrobium officinale polysaccharide triggers mitochondrial disorder to induce colon cancer cell death via ROS-AMPK-autophagy pathway.
- Source :
-
Carbohydrate polymers [Carbohydr Polym] 2021 Jul 15; Vol. 264, pp. 118018. Date of Electronic Publication: 2021 Apr 02. - Publication Year :
- 2021
-
Abstract
- The homeostasis between mitochondrial function and autophagy is crucial to the physiological activity of cancer cells, and its mechanism is conducive to the development of anti-tumor drugs. Here, we aimed to explore the effect and mechanism of Dendrobium officinale polysaccharide (DOP) on colon cancer cell line CT26. Our data showed that DOP significantly inhibited the proliferation of CT26 cells and elevated autophagy level. Moreover, DOP disrupted mitochondrial function through increasing reactive oxygen species (ROS) and reducing mitochondrial membrane potential (MMP), thereby impairing ATP biosynthesis, which activated AMPK/mTOR autophagy signaling. Intriguingly, the further experiments demonstrated that DOP-induced cytotoxicity, excessive autophagy and mitochondrial dysfunction were reversed after CT26 cells pretreated with antioxidant (N-acetyl-l-cysteine). Herein, these findings implied that DOP-induced mitochondrial dysfunction and cytotoxic autophagy repressed the propagation of CT26 cells via ROS-ATP-AMPK signaling, providing a new opinion for the study of antineoplastic drugs.<br /> (Copyright © 2021. Published by Elsevier Ltd.)
- Subjects :
- AMP-Activated Protein Kinases metabolism
Acetylcysteine pharmacology
Adenosine Triphosphate metabolism
Animals
Antineoplastic Agents, Phytogenic pharmacology
Antioxidants pharmacology
Apoptosis drug effects
Cell Line, Tumor
Cell Proliferation drug effects
Colonic Neoplasms metabolism
Membrane Potential, Mitochondrial drug effects
Mice
Reactive Oxygen Species metabolism
Signal Transduction
TOR Serine-Threonine Kinases metabolism
Autophagy drug effects
Cell Death drug effects
Colonic Neoplasms pathology
Dendrobium chemistry
Mitochondria drug effects
Polysaccharides pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1879-1344
- Volume :
- 264
- Database :
- MEDLINE
- Journal :
- Carbohydrate polymers
- Publication Type :
- Academic Journal
- Accession number :
- 33910741
- Full Text :
- https://doi.org/10.1016/j.carbpol.2021.118018