Back to Search
Start Over
A Novel Volume-Stable Collagen Matrix Induces Changes in the Behavior of Primary Human Oral Fibroblasts, Periodontal Ligament, and Endothelial Cells.
A Novel Volume-Stable Collagen Matrix Induces Changes in the Behavior of Primary Human Oral Fibroblasts, Periodontal Ligament, and Endothelial Cells.
- Source :
-
International journal of molecular sciences [Int J Mol Sci] 2021 Apr 14; Vol. 22 (8). Date of Electronic Publication: 2021 Apr 14. - Publication Year :
- 2021
-
Abstract
- The aim of the present study was to investigate the influence of a novel volume-stable collagen matrix (vCM) on early wound healing events including cellular migration and adhesion, protein adsorption and release, and the dynamics of the hemostatic system. For this purpose, we utilized transwell migration and crystal violet adhesion assays, ELISAs for quantification of adsorbed and released from the matrix growth factors, and qRT-PCR for quantification of gene expression in cells grown on the matrix. Our results demonstrated that primary human oral fibroblasts, periodontal ligament, and endothelial cells exhibited increased migration toward vCM compared to control cells that migrated in the absence of the matrix. Cellular adhesive properties on vCM were significantly increased compared to controls. Growth factors TGF-β1, PDGF-BB, FGF-2, and GDF-5 were adsorbed on vCM with great efficiency and continuously delivered in the medium after an initial burst release within hours. We observed statistically significant upregulation of genes encoding the antifibrinolytic thrombomodulin, plasminogen activator inhibitor type 1, thrombospondin 1, and thromboplastin, as well as strong downregulation of genes encoding the profibrinolytic tissue plasminogen activator, urokinase-type plasminogen activator, its receptor, and the matrix metalloproteinase 14 in cells grown on vCM. As a general trend, the stimulatory effect of the vCM on the expression of antifibrinolytic genes was synergistically enhanced by TGF-β1, PDGF-BB, or FGF-2, whereas the strong inhibitory effect of the vCM on the expression of profibrinolytic genes was reversed by PDGF-BB, FGF-2, or GDF-5. Taken together, our data strongly support the effect of the novel vCM on fibrin clot stabilization and coagulation/fibrinolysis equilibrium, thus facilitating progression to the next stages of the soft tissue healing process.
- Subjects :
- Animals
Becaplermin genetics
Cell Adhesion drug effects
Cell Movement drug effects
Collagen chemistry
Endothelial Cells drug effects
Fibrin genetics
Fibrinolysis drug effects
Fibroblast Growth Factor 2 genetics
Fibroblasts drug effects
Gene Expression Regulation, Developmental drug effects
Growth Differentiation Factor 5 genetics
Hemostasis drug effects
Heterografts
Humans
Mice
Mouth Mucosa growth & development
Periodontal Ligament growth & development
Primary Cell Culture
Transforming Growth Factor beta1 genetics
Collagen pharmacology
Mouth Mucosa drug effects
Periodontal Ligament drug effects
Regeneration genetics
Wound Healing genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1422-0067
- Volume :
- 22
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- International journal of molecular sciences
- Publication Type :
- Academic Journal
- Accession number :
- 33919968
- Full Text :
- https://doi.org/10.3390/ijms22084051