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Xanthohumol-Induced Rat Glioma C6 Cells Death by Triggering Mitochondrial Stress.
- Source :
-
International journal of molecular sciences [Int J Mol Sci] 2021 Apr 26; Vol. 22 (9). Date of Electronic Publication: 2021 Apr 26. - Publication Year :
- 2021
-
Abstract
- Aim: To investigate the underlying mechanisms of xanthohumol (XN) on the proliferation inhibition and death of C6 glioma cells.<br />Methods: To determine the effects of XN on C6 cells, cell proliferation and mortality after XN treatment were assessed by SRB assay and trypan blue assay respectively. Apoptotic rates were evaluated by flowcytometry after Annexin V-FITC/PI double staining. The influence of XN on the activity of caspase-3 was determined by Western blot (WB); and nuclear transposition of apoptosis-inducing factor (AIF) was tested by immunocytochemistry and WB. By MitoSOX <superscript>TM</superscript> staining, the mitochondrial ROS were detected. Mitochondrial function was also tested by MTT assay (content of succinic dehydrogenase), flow cytometry (mitochondrial membrane potential (MMP)-JC-1 staining; mitochondrial abundance-mito-Tracker green), immunofluorescence (MMP-JC-1 staining; mitochondrial morphology-mito-Tracker green), WB (mitochondrial fusion-fission protein-OPA1, mfn2, and DRP1; mitophagy-related proteins-Pink1, Parkin, LC3B, and P62), and high-performance liquid chromatography (HPLC) (energy charge). Finally, mitochondrial protein homeostasis of C6 cells after XN treatment with and without LONP1 inhibitor bortezomib was investigated by trypan blue assay (proliferative activity and mortality) and WB (mitochondrial protease LONP1). All cell morphology images were taken by a Leica Microsystems microscope.<br />Results: XN could lead to proliferation inhibition and death of C6 cells in a time- and dose-dependent manner and induce apoptosis of C6 cells through the AIF pathway. After long incubation of XN, mitochondria of C6 cells were seriously impaired, and mitochondria had a diffuse morphology and mitochondrial ROS were increased. The content of succinic dehydrogenase per cell was significantly decreased after XN insults of 24, 48, and 72 h. The energy charge was weakened after XN insult of 24 h. Furthermore, the MMP and mitochondrial abundance were significantly decreased; the protein expression levels of OPA1, mfn2, and DRP1 were down-regulated; and the protein expression levels of Pink1, Parkin, LC3B-II/LC3B-I, and p62 were up-regulated in long XN incubation times (24, 48, and 72 h). XN incubation with bortezomib for 48 h resulted in lower proliferative activity and higher mortality of C6 cells and caused the cell to have visible vacuoles. Moreover, the protein expression levels of LONP1 was up-regulated gradually as XN treatment time increased.<br />Conclusion: These data supported that XN could induce AIF pathway apoptosis of the rat glioma C6 cells by affecting the mitochondria.
- Subjects :
- Animals
Antineoplastic Agents pharmacology
Apoptosis drug effects
Apoptosis Inducing Factor metabolism
Caspase 3 analysis
Cell Death drug effects
Cell Line, Tumor
Cell Proliferation drug effects
China
Flavonoids metabolism
Glioma metabolism
Membrane Potential, Mitochondrial drug effects
Mitochondria drug effects
Mitochondrial Dynamics drug effects
Mitochondrial Proteins metabolism
Mitophagy drug effects
Neoplasm Invasiveness
Propiophenones metabolism
Rats
Reactive Oxygen Species analysis
Stress, Physiological drug effects
Flavonoids pharmacology
Glioma drug therapy
Mitochondria metabolism
Propiophenones pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1422-0067
- Volume :
- 22
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- International journal of molecular sciences
- Publication Type :
- Academic Journal
- Accession number :
- 33925918
- Full Text :
- https://doi.org/10.3390/ijms22094506