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Angiogenic potential of airbrushed fucoidan/polycaprolactone nanofibrous meshes.
Angiogenic potential of airbrushed fucoidan/polycaprolactone nanofibrous meshes.
- Source :
-
International journal of biological macromolecules [Int J Biol Macromol] 2021 Jul 31; Vol. 183, pp. 695-706. Date of Electronic Publication: 2021 Apr 28. - Publication Year :
- 2021
-
Abstract
- Implantation of biomaterials and hybrid constructs in tissue engineering approaches presents major limitations such as inflammatory reaction and the lack of vasculature integration. Therefore, new strategies are needed to enhance implant function, immune protection, and revascularization. In this work, we developed fibrous meshes composed of fucoidan (Fu), a sulfated polysaccharide extracted from brown algae, and polycaprolactone (PCL), a synthetic biodegradable polymer, using the airbrush technique. The chemical characterization by FTIR, EDS, and XPS confirmed the presence of the two polymers in the structure of airbrushed nanofibrous meshes (ANFM). Moreover, these nanofibrous exhibited good wettability and mechanical properties envisaging their application as templates for biomaterials and cell culture. The developed ANFM were directly cultured with human pulmonary microvascular endothelial (HPMEC-ST1.6R) cells for up to 7 days. Biological results demonstrated that ANFM comprising Fu promoted cellular attachment, spreading, and proliferation of human endothelial cells. The angiogenic potential of ANFM was further evaluated by onplantation of PCL and PCL/Fu ANFM in chick chorioallantoic membrane (CAM). In ovo and ex ovo results showed that the incorporation of Fu increased the pro-angiogenic potential of ANFM. Altogether, the results suggest that airbrush biocomposite meshes could be used as a biomaterial substrate to promote vascularization.<br /> (Copyright © 2021 Elsevier B.V. All rights reserved.)
- Subjects :
- Angiogenesis Inducing Agents chemistry
Animals
Cell Proliferation drug effects
Cell Survival drug effects
Cells, Cultured
Chick Embryo
Chorioallantoic Membrane drug effects
Endothelial Cells cytology
Endothelial Cells drug effects
Humans
Lung cytology
Lung drug effects
Microvessels cytology
Microvessels drug effects
Nanofibers
Polysaccharides chemistry
Surgical Mesh
Tissue Engineering
Angiogenesis Inducing Agents pharmacology
Chorioallantoic Membrane blood supply
Lung blood supply
Polyesters chemistry
Polysaccharides pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0003
- Volume :
- 183
- Database :
- MEDLINE
- Journal :
- International journal of biological macromolecules
- Publication Type :
- Academic Journal
- Accession number :
- 33932419
- Full Text :
- https://doi.org/10.1016/j.ijbiomac.2021.04.166