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Functional characterization of four TIR domain-containing adaptors, MyD88, TRIF, MAL, and SARM in mandarin fish Siniperca chuatsi.
- Source :
-
Developmental and comparative immunology [Dev Comp Immunol] 2021 Sep; Vol. 122, pp. 104110. Date of Electronic Publication: 2021 Apr 29. - Publication Year :
- 2021
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Abstract
- Toll/interleukin-1 receptor (TIR) domain-containing adaptors, serve as pivotal signal transduction molecules in Toll-like receptor (TLR) signalling pathway to mediate downstream signalling cascades. In this study, four TIR-domain containing adaptors, MyD88, TRIF, MAL and SARM, were identified in mandarin fish Siniperca chuatsi, and they all contain TIR domains, of which MyD88 and SARM had high sequence homology with their vertebrate homologues. The expression analysis at mRNA level indicated that these genes were ubiquitously distributed in different tissues, being high in immune- and mucosa-related tissues such as head-kidney and intestine. The transcripts of these adaptor genes were up-regulated by poly(I:C) and LPS stimulation in isolated head-kidney lymphocytes (HKLs) of mandarin fish. Fluorescence microscopy revealed that all these molecules were localized in cytoplasm, and further investigations showed that the over-expression of MyD88, TRIF and MAL activated the NF-κB, ISRE or type Ι IFN promoters and inhibited SVCV replication, whereas their antiviral effects were significantly impaired when co-transfected with SARM. It was also confirmed by co-immunoprecipitation (Co-IP) that SARM interacts separately with MyD88, TRIF and MAL, and MAL interacts with MyD88. However, the regulatory mechanisms of these adaptors involved in signalling pathways of different TLRs should be of interest for further research.<br /> (Copyright © 2021 Elsevier Ltd. All rights reserved.)
- Subjects :
- Adaptor Proteins, Vesicular Transport genetics
Animals
Armadillo Domain Proteins genetics
Cell Line
Fish Diseases immunology
Fish Diseases virology
HEK293 Cells
Head Kidney cytology
Head Kidney immunology
Humans
Interferon Type I genetics
Interferon Type I immunology
Lipopolysaccharides immunology
Membrane Glycoproteins genetics
Membrane Glycoproteins metabolism
Myeloid Differentiation Factor 88 genetics
NF-kappa B metabolism
Perciformes virology
Poly I-C immunology
Protein Domains
Receptors, Interleukin-1 genetics
Signal Transduction physiology
Transcriptional Activation
Virus Replication immunology
Adaptor Proteins, Vesicular Transport metabolism
Armadillo Domain Proteins metabolism
Lymphocytes immunology
Myeloid Differentiation Factor 88 metabolism
Perciformes immunology
Receptors, Interleukin-1 metabolism
Rhabdoviridae immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0089
- Volume :
- 122
- Database :
- MEDLINE
- Journal :
- Developmental and comparative immunology
- Publication Type :
- Academic Journal
- Accession number :
- 33933533
- Full Text :
- https://doi.org/10.1016/j.dci.2021.104110