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Bilateral NIRS measurements of muscle mitochondrial capacity: Feasibility and repeatability.

Authors :
Hanna R
Gosalia J
Demalis A
Hobson Z
McCully KK
Irving BA
Mookerjee S
Vairo GL
Proctor DN
Source :
Physiological reports [Physiol Rep] 2021 Apr; Vol. 9 (8), pp. e14826.
Publication Year :
2021

Abstract

Background: Non-invasive determination of mitochondrial capacity via near infrared spectroscopy (NIRS) typically involves voluntary exercise of a single muscle group followed by as many as 26 brief ischemic cuff occlusions to determine a single recovery rate constant (k).<br />Purpose: To determine the within- and between-visit repeatability of a shortened bilateral NIRS protocol, and to establish the feasibility of hamstring k measurements.<br />Methods: Sixteen young (eight women, eight men; 22 ± 3 years) active adults underwent a bilateral electrical stimulation protocol in which multiple (n = 4) measurements of k for the vastus lateralis (VL) and medial hamstring (MH) muscles were determined on two visits. Repeatability (CV% and intraclass correlations, ICC) and equivalency across visits were assessed for both muscles.<br />Results: Mean k values in the VL were consistent with published values and within-visit ICCs were moderately high for both muscles in both sexes. In men, average k values on visit 2 were within 1% (VL muscle) and 5% (MH muscle) of the values on visit 1 (all p > 0.78). In women, average k values were 10%-15% lower on visit 2 (p = 0.01 and p = 0.15 for MH and VL) with the largest between-visit differences in a subset of participants with the most days between visits.<br />Conclusions: This bilateral NIRS protocol is time efficient and provides valid estimates of k in both sexes and muscle groups with acceptable within-visit repeatability. Lower than expected between-visit repeatability in some participants reinforces the need for further investigation of this newly developed protocol to identify and control for experimental and behavioral sources of variation.<br /> (© 2021 The Authors. Physiological Reports published by Wiley Periodicals LLC on behalf of The Physiological Society and the American Physiological Society.)

Details

Language :
English
ISSN :
2051-817X
Volume :
9
Issue :
8
Database :
MEDLINE
Journal :
Physiological reports
Publication Type :
Academic Journal
Accession number :
33945230
Full Text :
https://doi.org/10.14814/phy2.14826