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Prevalent, protective, and convergent IgG recognition of SARS-CoV-2 non-RBD spike epitopes.
- Source :
-
Science (New York, N.Y.) [Science] 2021 Jun 04; Vol. 372 (6546), pp. 1108-1112. Date of Electronic Publication: 2021 May 04. - Publication Year :
- 2021
-
Abstract
- The molecular composition and binding epitopes of the immunoglobulin G (IgG) antibodies that circulate in blood plasma after severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection are unknown. Proteomic deconvolution of the IgG repertoire to the spike glycoprotein in convalescent subjects revealed that the response is directed predominantly (>80%) against epitopes residing outside the receptor binding domain (RBD). In one subject, just four IgG lineages accounted for 93.5% of the response, including an amino (N)-terminal domain (NTD)-directed antibody that was protective against lethal viral challenge. Genetic, structural, and functional characterization of a multidonor class of "public" antibodies revealed an NTD epitope that is recurrently mutated among emerging SARS-CoV-2 variants of concern. These data show that "public" NTD-directed and other non-RBD plasma antibodies are prevalent and have implications for SARS-CoV-2 protection and antibody escape.<br /> (Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.)
- Subjects :
- Animals
Antibodies, Monoclonal blood
Antibodies, Monoclonal chemistry
Antibodies, Monoclonal immunology
Antibodies, Neutralizing blood
Antibodies, Neutralizing chemistry
Antibodies, Viral blood
Antibodies, Viral chemistry
Antibody Affinity
COVID-19 prevention & control
Epitopes immunology
Humans
Immune Evasion
Immunoglobulin G blood
Immunoglobulin G chemistry
Immunoglobulin Heavy Chains immunology
Immunoglobulin Variable Region immunology
Mice
Mice, Inbred BALB C
Mutation
Protein Domains
Proteomics
SARS-CoV-2 genetics
Spike Glycoprotein, Coronavirus chemistry
Spike Glycoprotein, Coronavirus genetics
Antibodies, Neutralizing immunology
Antibodies, Viral immunology
COVID-19 immunology
Immunoglobulin G immunology
SARS-CoV-2 immunology
Spike Glycoprotein, Coronavirus immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1095-9203
- Volume :
- 372
- Issue :
- 6546
- Database :
- MEDLINE
- Journal :
- Science (New York, N.Y.)
- Publication Type :
- Academic Journal
- Accession number :
- 33947773
- Full Text :
- https://doi.org/10.1126/science.abg5268