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DIA-MS E to Study Microglial Function in Schizophrenia.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2228, pp. 341-352. - Publication Year :
- 2021
-
Abstract
- Here, we describe a proteomic pipeline to use a human microglial cell line as a biological model to study schizophrenia. In order to maximize the proteome coverage, we apply two-dimensional liquid chromatography coupled with ultra-definition MS <superscript>E</superscript> mass spectrometry (LC-UDMS <superscript>E</superscript> ) using a data-independent acquisition (DIA) approach, with an optimization of drift time collision energy.
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 2228
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 33950502
- Full Text :
- https://doi.org/10.1007/978-1-0716-1024-4_24