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Effect of MicroRNA145 on the multidrug resistance gene of ulcerative colitis in rats.
- Source :
-
Life sciences [Life Sci] 2021 Aug 01; Vol. 278, pp. 119603. Date of Electronic Publication: 2021 May 11. - Publication Year :
- 2021
-
Abstract
- Multidrug resistance gene (MDR1a) and P-glycoprotein (P-gp) play an important role in the development of ulcerative colitis (UC) and influence the therapeutic effect of glucocorticoids, which may lead to drug resistance mechanically. UC may be related to miR-145 to some extent, and the relationship still needs further exploration. In this study we found that the expression of miR-145 was downregulated in the colonic tissues of rats with Dextran sodium sulfate (DSS)-induced UC. Also, the expression of MDR1a in colon tissues of each group negatively correlated with the expression of miR-145 in rats. The change in the plasma peak concentration (C <subscript>max</subscript> ) in each group positively related to the miR-145 level. Mechanistically, miR-145 negatively regulated the expression and function of P-gp via acting directly on the 3'-UTR of MDR1 mRNA. Overall, these results indicated that miR-145 had a protective effect on the colorectal mucosa, and its downregulation may enhance the expression and function of MDR1a and P-gp, promoting the occurrence and development of UC. The downregulation of miR-145 reduced the drug sensitivity of 5-aminosalicylic acid (5-ASA) and glucocorticoids in treating UC, indicating that miR-145 might be a potential therapeutic target for UC.<br /> (Copyright © 2021 Elsevier Inc. All rights reserved.)
- Subjects :
- 3' Untranslated Regions
Animals
Colitis, Ulcerative pathology
Gene Expression Regulation
Genes, MDR
Male
RNA, Messenger genetics
Rats, Sprague-Dawley
Rats
ATP Binding Cassette Transporter, Subfamily B genetics
ATP Binding Cassette Transporter, Subfamily B, Member 1 genetics
Colitis, Ulcerative genetics
MicroRNAs genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0631
- Volume :
- 278
- Database :
- MEDLINE
- Journal :
- Life sciences
- Publication Type :
- Academic Journal
- Accession number :
- 33984358
- Full Text :
- https://doi.org/10.1016/j.lfs.2021.119603