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Lysionotin induces apoptosis of hepatocellular carcinoma cells via caspase-3 mediated mitochondrial pathway.
- Source :
-
Chemico-biological interactions [Chem Biol Interact] 2021 Aug 01; Vol. 344, pp. 109500. Date of Electronic Publication: 2021 May 11. - Publication Year :
- 2021
-
Abstract
- As the sixth most prevalent cancer, liver cancer has been reported as the second cause of cancer-induced deaths globally. Lysionotin, a flavonoid compound widely distributed in Lysionotus pauciflorus Maxim, has attracted considerable attention due to its multiple biological activities. The present study analyzes the anti-liver cancer effects of lysionotin in cells and mouse models. In HepG2 and SMMC-7721 cells, lysionotin significantly reduced the viability of cells, inhibited cell proliferation and migration, enhanced cell apoptosis, promoted the increase of intracellular reactive oxygen species (ROS) levels, decreased mitochondrial membrane potential (MMP), and alternated the content of apoptosis-related proteins. In HepG2-and SMMC-7721-xenograft tumor mouse models, lysionotin inhibited tumor growth, reduced the expression levels of anti-apoptotic proteins and enhanced the expression levels of pro-apoptotic proteins in tumor tissues. Additionally, the pre-treatment of Ac-DEVD-CHO, an inhibitor of caspase-3, strongly restored the low cell viability, the enhanced apoptosis rate, the dissipation of MMP caused by lysionotin exposure, as well as prevented the lysionotin-caused enhancement on expressions of apoptosis related proteins, especially cleaved poly (ADP-ribose) polymerase (PARP), Fas Ligand (FasL), cleaved caspase-3 and Bax in both HepG2 and SMMC-7721 cells. Altogether, lysionotin showed significant anti-liver cancer effects related to caspase-3 mediated mitochondrial apoptosis.<br /> (Copyright © 2021. Published by Elsevier B.V.)
- Subjects :
- Animals
Carcinoma, Hepatocellular pathology
Caspase 3 metabolism
Cell Line, Tumor
Cell Proliferation drug effects
Humans
Liver Neoplasms pathology
Male
Membrane Potential, Mitochondrial drug effects
Mice, Inbred BALB C
Reactive Oxygen Species metabolism
Xenograft Model Antitumor Assays
Mice
Antineoplastic Agents therapeutic use
Apoptosis drug effects
Carcinoma, Hepatocellular drug therapy
Flavones therapeutic use
Liver Neoplasms drug therapy
Mitochondria drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1872-7786
- Volume :
- 344
- Database :
- MEDLINE
- Journal :
- Chemico-biological interactions
- Publication Type :
- Academic Journal
- Accession number :
- 33989594
- Full Text :
- https://doi.org/10.1016/j.cbi.2021.109500