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Rare Missense Functional Variants at COL4A1 and COL4A2 in Sporadic Intracerebral Hemorrhage.

Authors :
Chung J
Hamilton G
Kim M
Marini S
Montgomery B
Henry J
Cho AE
Brown DL
Worrall BB
Meschia JF
Silliman SL
Selim M
Tirschwell DL
Kidwell CS
Kissela B
Greenberg SM
Viswanathan A
Goldstein JN
Langefeld CD
Rannikmae K
Sudlow CLM
Samarasekera N
Rodrigues M
Al-Shahi Salman R
Prendergast JGD
Harris SE
Deary I
Woo D
Rosand J
Van Agtmael T
Anderson CD
Source :
Neurology [Neurology] 2021 Jul 19; Vol. 97 (3), pp. e236-e247. Date of Electronic Publication: 2021 Jul 19.
Publication Year :
2021

Abstract

Objective: To test the genetic contribution of rare missense variants in COL4A1 and COL4A2 in which common variants are genetically associated with sporadic intracerebral hemorrhage (ICH), we performed rare variant analysis in multiple sequencing data for the risk for sporadic ICH.<br />Methods: We performed sequencing across 559 Kbp at 13q34 including COL4A1 and COL4A2 among 2,133 individuals (1,055 ICH cases; 1,078 controls) in United States-based and 1,381 individuals (192 ICH cases; 1,189 controls) from Scotland-based cohorts, followed by sequence annotation, functional impact prediction, genetic association testing, and in silico thermodynamic modeling.<br />Results: We identified 107 rare nonsynonymous variants in sporadic ICH, of which 2 missense variants, rs138269346 (COL4A1 <superscript>I110T</superscript> ) and rs201716258 (COL4A2 <superscript>H203L</superscript> ), were predicted to be highly functional and occurred in multiple ICH cases but not in controls from the United States-based cohort. The minor allele of rs201716258 was also present in Scottish patients with ICH, and rs138269346 was observed in 2 ICH-free controls with a history of hypertension and myocardial infarction. Rs138269346 was nominally associated with nonlobar ICH risk ( p = 0.05), but not with lobar ICH ( p = 0.08), while associations between rs201716258 and ICH subtypes were nonsignificant ( p > 0.12). Both variants were considered pathogenic based on minor allele frequency (<0.00035 in European populations), predicted functional impact (deleterious or probably damaging), and in silico modeling studies (substantially altered physical length and thermal stability of collagen).<br />Conclusions: We identified rare missense variants in COL4A1 / A2 in association with sporadic ICH. Our annotation and simulation studies suggest that these variants are highly functional and may represent targets for translational follow-up.<br /> (Copyright © 2021 The Author(s). Published by Wolters Kluwer Health, Inc. on behalf of the American Academy of Neurology.)

Details

Language :
English
ISSN :
1526-632X
Volume :
97
Issue :
3
Database :
MEDLINE
Journal :
Neurology
Publication Type :
Academic Journal
Accession number :
34031201
Full Text :
https://doi.org/10.1212/WNL.0000000000012227