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Establishment and characterization of an ovarian yolk sac tumor patient-derived xenograft model.
- Source :
-
Pediatric surgery international [Pediatr Surg Int] 2021 Aug; Vol. 37 (8), pp. 1031-1040. Date of Electronic Publication: 2021 May 24. - Publication Year :
- 2021
-
Abstract
- Objective: The lack of appropriate preclinical models of ovarian yolk sac tumor (OYST) is currently hindering the pursuit of new methods of treatment and investigation of the pathogenesis of the disease. We developed and characterized an OYST patient-derived xenograft (PDX) model in this study.<br />Methods: Tumor fragments from a patient with an OYST were implanted subcutaneously into BALB/c Nude mice. Engrafted xenografts were compared with the original tumor according to histology, immunohistochemistry, humanized identified, and drug efficacy testing with in vivo treatment programs.<br />Results: There was a high degree of histologic and immunohistochemical (IHC) resemblance between the established PDX model and its corresponding human tumors. Bleomycin, etoposide, and cisplatin (JEB) chemotherapy regimens were effective in clinical patients and were effective in the OYST PDX model; therefore, the effect of PDX intervention was consistent with clinical outcomes of OYSTs.<br />Conclusion: We have successfully established an OYST PDX model. This OYST model preserves the basic molecular features of the primary human tumor, thereby providing a valuable method to preclinically evaluate new treatments and explore disease pathogenesis.
- Subjects :
- Animals
Antineoplastic Agents therapeutic use
Bleomycin therapeutic use
Cisplatin therapeutic use
Disease Models, Animal
Endodermal Sinus Tumor drug therapy
Endodermal Sinus Tumor genetics
Etoposide therapeutic use
Female
Heterografts transplantation
Humans
Mice
Mice, Inbred BALB C
Mice, Nude
Neoplasms, Germ Cell and Embryonal drug therapy
Neoplasms, Germ Cell and Embryonal genetics
Ovarian Neoplasms drug therapy
Ovarian Neoplasms genetics
Endodermal Sinus Tumor pathology
Neoplasms, Germ Cell and Embryonal pathology
Ovarian Neoplasms pathology
Transplantation, Heterologous methods
Xenograft Model Antitumor Assays methods
Subjects
Details
- Language :
- English
- ISSN :
- 1437-9813
- Volume :
- 37
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Pediatric surgery international
- Publication Type :
- Academic Journal
- Accession number :
- 34031745
- Full Text :
- https://doi.org/10.1007/s00383-021-04895-1