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Cytosolic replication in epithelial cells fuels intestinal expansion and chronic fecal shedding of Salmonella Typhimurium.
- Source :
-
Cell host & microbe [Cell Host Microbe] 2021 Jul 14; Vol. 29 (7), pp. 1177-1185.e6. Date of Electronic Publication: 2021 May 26. - Publication Year :
- 2021
-
Abstract
- Persistent and intermittent fecal shedding, hallmarks of Salmonella infections, are important for fecal-oral transmission. In the intestine, Salmonella enterica serovar Typhimurium (STm) actively invades intestinal epithelial cells (IECs) and survives in the Salmonella-containing vacuole (SCV) and the cell cytosol. Cytosolic STm replicate rapidly, express invasion factors, and induce extrusion of infected epithelial cells into the intestinal lumen. Here, we engineered STm that self-destruct in the cytosol (STm <superscript>CytoKill</superscript> ), but replicates normally in the SCV, to examine the role of cytosolic STm in infection. Intestinal expansion and fecal shedding of STm <superscript>CytoKill</superscript> are impaired in mouse models of infection. We propose a model whereby repeated rounds of invasion, cytosolic replication, and release of invasive STm from extruded IECs fuels the high luminal density required for fecal shedding.<br />Competing Interests: Declaration of interests The authors declare no competing interests.<br /> (Published by Elsevier Inc.)
- Subjects :
- Animals
Female
HeLa Cells
Humans
Intestines microbiology
Male
Mice
Mice, Inbred C57BL
Salmonella typhimurium genetics
Salmonella typhimurium growth & development
Vacuoles microbiology
Cytosol microbiology
Epithelial Cells microbiology
Feces microbiology
Salmonella Infections microbiology
Salmonella typhimurium physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1934-6069
- Volume :
- 29
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Cell host & microbe
- Publication Type :
- Academic Journal
- Accession number :
- 34043959
- Full Text :
- https://doi.org/10.1016/j.chom.2021.04.017