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MicroRNA-224 modulates chemosensitivity of breast cancer cells to docetaxel by apoptosis inhibitor 5.
- Source :
-
Journal of B.U.ON. : official journal of the Balkan Union of Oncology [J BUON] 2021 Mar-Apr; Vol. 26 (2), pp. 450-458. - Publication Year :
- 2021
-
Abstract
- Purpose: The view that microRNA-224 (miR-224) may lead to tumorigenesis has been accepted in many studies. However, its role remains unclear in modulating the chemosensitivity of breast cancer cells to docetaxel (DOC). Thus, the aim of this study was to estimate what's the role of miR-224 in the chemosensitivity of breast cancer cells to DOC.<br />Methods: The role of miR-224 in breast cancer cells was analyzed using CCK-8 assay, real-time PCR, flow cytometry assay and Western blot. Dual-luciferase reporter assay and API-5-siRNA technology were performed to analyze the association between miR-224 and Apoptosis inhibitor 5 (API-5).<br />Results: Overexpression of miR-224 could significantly decrease the chemosensitivity of MCF-7 breast cancer cells to DOC. The luciferase activity of MCF-7/DOC cells containing wild-type 3'UTR of API-5 could be inhibited by miR-224 mimics. Similarly, the chemoresistance of MCF-7 cells to DOC induced by miR-224 mimics could be partially reversed by API-5-siRNA.<br />Conclusion: An inverse association between miR-224 and API-5 in breast cancer cells was revealed. Dysregulation of miR-224 plays a vital role in the acquired DOC resistance of breast cancer and at least partially via targeting API-5.
- Subjects :
- Antineoplastic Agents pharmacology
Apoptosis drug effects
Breast Neoplasms pathology
Female
Humans
Transfection
Antineoplastic Agents therapeutic use
Apoptosis Regulatory Proteins metabolism
Breast Neoplasms drug therapy
Docetaxel pharmacology
MicroRNAs metabolism
Nuclear Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2241-6293
- Volume :
- 26
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of B.U.ON. : official journal of the Balkan Union of Oncology
- Publication Type :
- Academic Journal
- Accession number :
- 34076992