Pseudomonas paraversuta sp. nov. isolated from refrigerated dry-aged beef.

A polyphasic approach was applied to investigate the diversity of microbiota that evolved during cold storage beef ripening. Isolate V4/DAB/S4/2a ^T with a unique BOX-rep-PCR fingerprint profile revealed more than 99 % nucleotide identities upon pairwise comparisons of 16S rDNA sequences from the type strains Pseudomonas versuta DSM 101070 ^T , Pseudomonas saxonica DSM 108989 ^T , Pseudomonas deceptionensis DSM 26521 ^T and Pseudomonas weihenstephanensis DSM 29166 ^T , placing it within the Pseudomonas fragi / lundensis branch of the genus Pseudomonas . Additional rpoB based comparison revealed P. versuta DSM 101070 ^T as the nearest relative, with 98.5 % nucleotide identity. Calculation of ANIb values of the V4/DAB/S4/2a ^T draft genome identified P. versuta DSM 101070 ^T with 90.1 %, P. deceptionensis DSM 26521 ^T with 85.1 %, P. fragi DSM 3456 ^T with 84.4 %, Pseudomonas psychrophila DSM 17535 ^T and Pseudomonas bubulae DSM 107389 ^T with 84.2 % similarities each. Pairwise genome-to-genome distance calculations [digital DNA-DNA hybridization (dDDH)] resulted in values of 47.1, 35.1, 34.8, 34.2 and 34.1 %, respectively. A second isolate was detected years later in ground beef and showed ANIb values of 99.3 % and dDDH of 96.1 % relatedness to V4/DAB/S4/2a ^T . The DNA G+C content was 58.6 mol% for both isolates. The predominant cellular fatty acids of V4/DAB/S4/2a ^T were C _16 : 0 , C _18 : 1 ω7 c , C _17 : 0 cyclo and a summed feature containing C _16 : 1 ω7 c and/or C _15 : 0 iso 2-OH. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol, the major respiratory quinone was Q9, with a small portion of Q8. The combined data on genotypic and phenotypic features support the proposal of a novel species, for which the name Pseudomonas paraversuta sp. nov. is proposed. The type strain is V4/DAB/S4/2a ^T (=DSM 111361 ^T =LMG 31844 ^T ) and a second isolate is UBT376 (=DSM 111360=LMG 31845).