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Raman spectroscopy for guidance of vulvar cancer surgery: a pilot study.

Authors :: van Doorn HC
Barroso EM
Koljenović S
Ewing-Graham PC
Soares MRN
van de Berg NJ
Schut TCB
Puppels GJ
Source :: Biomedical optics express [Biomed Opt Express] 2021 Apr 28; Vol. 12 (5), pp. 3008-3020. Date of Electronic Publication: 2021 Apr 28 (Print Publication: 2021).
Publication Year :: 2021
Abstract: For vulvar squamous cell carcinoma (VSCC), the mainstay of treatment is surgical removal with tumour-free margins. Surgeons still operate without objective tools that provide margin-status. This study assesses Raman spectroscopy potentiality for distinguishing ex-vivo VSCC from healthy tissue in 11 patients. Grid-based Raman maps were obtained from processed spectra. Water content and C-H band ratio (2,910-2,966 cm <superscript>-1</superscript> / 2810-2890 cm <superscript>-1</superscript> ) were calculated per spectrum and used as linear discriminant parameters. Healthy tissue was differentiated from VSCC with 0.90 discriminative power, 0.79 sensitivity and 0.86 specificity.This is an important step towards the development of objective tools for VSCC surgical guidance.<br />Competing Interests: S. Koljenović, and G.J. Puppels have ownership interest (including patents) in RiverD International BV. T.C. Bakker Schut, M. Rosa N. Soares are employees of RiverD International BV. M. Rosa N. Soares is also employee of RiverD International BV. G.J. Puppels is employed with RiverD International BV as a CTO & Managing Director. No potential conflicts of interest were disclosed by the other authors.<br /> (© 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.)