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N- trans -Feruloyloctopamine Wakes Up BBC3, DDIT3, CDKN1A, and NOXA Signals to Accelerate HCC Cell Apoptosis.
- Source :
-
Analytical cellular pathology (Amsterdam) [Anal Cell Pathol (Amst)] 2021 May 22; Vol. 2021, pp. 1560307. Date of Electronic Publication: 2021 May 22 (Print Publication: 2021). - Publication Year :
- 2021
-
Abstract
- N- trans -Feruloyloctopamine (FO), a natural compound, was reported in our previous study to inhibit a tumor cell malignant phenotype by AKT- and EMT-related signals and might be used as a promising drug for HCC treatment. However, the specific targets and detailed mechanisms still need to be clarified. Screening with RNA-Seq in Huh7 cells treated with FO revealed that 317 genes were modulated, of which 188 genes were upregulated and 129 genes were downregulated. Real-time cell analyzer and flow cytometry data reveal that tumor cell proliferation and apoptosis were impacted by FO. DAVID bioinformatic data showed that most of the biological process GO terms are related to proliferation and apoptosis. KEGG enrichment analysis showed that FO mainly regulates PI3K-AKT- and apoptosis-related signals, in which BBC3, DDIT3, NOXA, and CDKN1A on the surface serve as the novel targets of FO inducing HCC cell apoptosis. The result implied that FO might exacerbate HCC cell apoptosis by regulating BBC3, DDIT3, CDKN1A, and NOXA signals. The obstacle effect of FO can provide new targets and new credibility for the treatment of liver cancer.<br />Competing Interests: All authors declare that they have no conflicts of interest.<br /> (Copyright © 2021 Bin Ma et al.)
- Subjects :
- Apoptosis genetics
Carcinoma, Hepatocellular metabolism
Carcinoma, Hepatocellular pathology
Cell Line, Tumor
Cell Proliferation drug effects
Cell Proliferation genetics
Coumaric Acids chemistry
Cyclin-Dependent Kinase Inhibitor p21 genetics
Gene Expression Profiling
Gene Expression Regulation, Neoplastic drug effects
Humans
Liver Neoplasms metabolism
Liver Neoplasms pathology
Molecular Structure
Octopamine chemistry
Octopamine pharmacology
Phosphatidylinositol 3-Kinases metabolism
Proto-Oncogene Proteins genetics
Proto-Oncogene Proteins c-akt metabolism
Proto-Oncogene Proteins c-bcl-2 genetics
RNA-Seq methods
Signal Transduction genetics
Transcription Factor CHOP genetics
Apoptosis drug effects
Apoptosis Regulatory Proteins genetics
Carcinoma, Hepatocellular genetics
Coumaric Acids pharmacology
Liver Neoplasms genetics
Octopamine analogs & derivatives
Signal Transduction drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 2210-7185
- Volume :
- 2021
- Database :
- MEDLINE
- Journal :
- Analytical cellular pathology (Amsterdam)
- Publication Type :
- Academic Journal
- Accession number :
- 34123711
- Full Text :
- https://doi.org/10.1155/2021/1560307