Back to Search
Start Over
Establishment of Rat Models of Different Pathological Types of Hypersensitivity Pneumonitis Using Pigeon Droppings.
- Source :
-
International archives of allergy and immunology [Int Arch Allergy Immunol] 2021; Vol. 182 (11), pp. 1017-1025. Date of Electronic Publication: 2021 Jun 17. - Publication Year :
- 2021
-
Abstract
- Background: The pathogenesis and pulmonary histopathological characteristics of hypersensitivity pneumonitis (HP) are not yet fully understood. Therefore, we established animal models of HP of different stages, aiming to provide support for research on this disease.<br />Methods: We established rat models of pigeon breeder's lung of different pathological types by creating freeze-dried allergen powder from fresh pigeon feathers, dander, and other droppings. Freeze-dried allergen powder suspensions of pigeon droppings were used to establish 2 rat models of HP, one by aerosol inhalation and one by airway instillation, and the rats were sacrificed after different lengths of time to observe the pathological changes in their lung tissues.<br />Results: By the 40th week after allergen inhalation, granulomas were the main changes in the model, without fibrotic changes. When using airway instillation to establish the model, at the 20th week, group 1 (low dose + twice/week) and group 2 (medium dose + twice/week) showed granuloma changes, but no fibrosis; group 3 (high dose + once/week) and group 4 (high dose + twice/week) both showed obvious pulmonary fibrotic changes, but the death rate of rats in group 4 was greater.<br />Conclusions: Both aerosol inhalation and airway instillation of freeze-dried pigeon allergen powder can successfully establish an HP model. The airway instillation method can cause pulmonary fibrotic changes in a short time, and the pulmonary pathological changes of animal models manifest with an obvious time-dose effect.<br /> (© 2021 S. Karger AG, Basel.)
- Subjects :
- Administration, Inhalation
Aerosols
Allergens administration & dosage
Animals
Columbidae immunology
Dander immunology
Feathers immunology
Feces
Female
Freeze Drying
Granuloma immunology
Granuloma pathology
Lung immunology
Lung pathology
Lymphocytes immunology
Macrophages immunology
Male
Powders
Pulmonary Fibrosis immunology
Pulmonary Fibrosis pathology
Rats, Sprague-Dawley
Rats
Bird Fancier's Lung immunology
Bird Fancier's Lung pathology
Disease Models, Animal
Subjects
Details
- Language :
- English
- ISSN :
- 1423-0097
- Volume :
- 182
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- International archives of allergy and immunology
- Publication Type :
- Academic Journal
- Accession number :
- 34139709
- Full Text :
- https://doi.org/10.1159/000516233