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Microcarrier-Supported Culture of Chondrocytes in Continuously Rocked Disposable Bioreactor.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2436, pp. 145-156. - Publication Year :
- 2022
-
Abstract
- Disposable wave-assisted bioreactors are devices originally designed for scaling-up cultures of extremely fragile animal cells. In such bioreactors, agitation is achieved by continuous horizontal oscillations of disposable culture bag-like container fixed in a rocker unit. The continuous rocking movement of the container induces waves in the two-phase (i.e., gas-liquid) culture system composed of CO <subscript>2</subscript> -enriched air and aqueous culture medium. Such continuously oscillating devices can be utilized for supporting homogeneity in systems for in vitro propagation of animal anchorage-dependent, that is, adherent, cells, like CP5 chondrocytes cells. As most of in vitro cultured cells exhibit anchorage-dependency toward solid surface, the suitable interface can be provided by beads of microcarriers made of polymers and characterized by large surface-to-volume ratio. This chapter describes a methodology for efficient propagation of CP5 chondrocytes on Cytodex 3 microcarriers performed in ReadyToProcess WAVE 25 disposable bioreactor, as well as all useful procedures for daily monitoring the growth of CP5 chondrocytes.<br /> (© 2021. Springer Science+Business Media, LLC.)
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 2436
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 34155605
- Full Text :
- https://doi.org/10.1007/7651_2021_411