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Pseudogenes as Competitive Endogenous RNAs: Target Prediction and Validation.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2324, pp. 115-129. - Publication Year :
- 2021
-
Abstract
- Pseudogenes may regulate expression of their parental genes as well as other protein-coding genes through various mechanisms. One such mechanism is the ability to act as competitive endogenous RNA (ceRNA) and participate in microRNA-mediated cross-regulation. Here, we outline how to predict the targets of pseudogene ceRNAs bioinformatically and how to validate them experimentally.
- Subjects :
- 3' Untranslated Regions
Animals
Cell Line
Gene Silencing
Genomics methods
Humans
MicroRNAs metabolism
RNA, Messenger genetics
RNA, Messenger metabolism
RNA, Small Interfering
Transfection
Computational Biology methods
MicroRNAs genetics
Pseudogenes genetics
Regulatory Sequences, Ribonucleic Acid genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 2324
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 34165712
- Full Text :
- https://doi.org/10.1007/978-1-0716-1503-4_8