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Exploration of the Function of Ginsenoside RD Attenuates Lipopolysaccharide-Induced Lung Injury: A Study of Network Pharmacology and Experimental Validation.
- Source :
-
Shock (Augusta, Ga.) [Shock] 2022 Feb 01; Vol. 57 (2), pp. 212-220. - Publication Year :
- 2022
-
Abstract
- Objective: Ginsenoside Rd (GSRd) displays a variety of pharmacological effects. However, the underlying role in acute lung injury (ALI) is not clear. In this study, the protective effect of GSRd on lipopolysaccharide (LPS)-induced ALI is investigated to explore the potential mechanisms.<br />Methods: GSRd-target-ALI-related gene set was constructed. And bioinformatics tools were used to discover the potential mechanism. We observed the survival of subjects for 72 h. In addition, male BALB/c mice were intraperitoneal injected with GSRd (25 and 50 mg/kg) after received one intratracheal instillation of LPS. Inflammatory changes, oxidative stress, and phosphorylation were assessed to study the biological effects.<br />Results: A total of 245 interaction genes were collected. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were enriched in immune-inflammatory system. Among them, PI3K-Akt signaling pathway was the highest-ranked pathway of inflammatory response. In vivo study, it was found that GSRd improved survival in endotoxemic mice and inhibited the major characteristic of ALI. And the p-PI3K and p-Akt expression was significantly decreased by GSRd treatment.<br />Conclusion: GSRd could protect mice against LPS-induced ALI effectively by inhibiting the PI3K-Akt signaling pathway.<br />Competing Interests: None of the authors have any conflicts of interest to declare.<br /> (Copyright © 2021 by the Shock Society.)
- Subjects :
- Acute Lung Injury chemically induced
Acute Lung Injury mortality
Animals
Ginsenosides pharmacology
Lipopolysaccharides administration & dosage
Male
Mice
Mice, Inbred BALB C
Phosphatidylinositol 3-Kinases drug effects
Signal Transduction drug effects
Survival Rate
Acute Lung Injury drug therapy
Ginsenosides therapeutic use
Subjects
Details
- Language :
- English
- ISSN :
- 1540-0514
- Volume :
- 57
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Shock (Augusta, Ga.)
- Publication Type :
- Academic Journal
- Accession number :
- 34172615
- Full Text :
- https://doi.org/10.1097/SHK.0000000000001824