Intestinal multicellular organoids to study colorectal cancer.

Authors :: Idris M
Alves MM
Hofstra RMW
Mahe MM
Melotte V
Source :: Biochimica et biophysica acta. Reviews on cancer [Biochim Biophys Acta Rev Cancer] 2021 Dec; Vol. 1876 (2), pp. 188586. Date of Electronic Publication: 2021 Jun 30.
Publication Year :: 2021
Abstract: Modeling colorectal cancer (CRC) using organoids has burgeoned in the last decade, providing enhanced in vitro models to study the development and possible treatment options for this type of cancer. In this review, we describe both normal and CRC intestinal organoid models and their utility in the cancer research field. Besides highlighting studies that develop epithelial CRC organoid models, i.e. organoids without tumor microenvironment (TME) cellular components, we emphasize on the need for TME in CRC modeling, to help reduce translational disparities in this area. Also, we discuss the utilization of CRC organoids derived from pluripotent stem cells, as well as their potential to be used in cancer research. Finally, limitations and challenges in the current CRC organoids field, are discussed.<br /> (Copyright © 2021 The Author(s). Published by Elsevier B.V. All rights reserved.)