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PNKP is required for maintaining the integrity of progenitor cell populations in adult mice.
- Source :
-
Life science alliance [Life Sci Alliance] 2021 Jul 05; Vol. 4 (9). Date of Electronic Publication: 2021 Jul 05 (Print Publication: 2021). - Publication Year :
- 2021
-
Abstract
- DNA repair proteins are critical to the maintenance of genomic integrity. Specific types of genotoxic factors, including reactive oxygen species generated during normal cellular metabolism or as a result of exposure to exogenous oxidative agents, frequently leads to "ragged" single-strand DNA breaks. The latter exhibits abnormal free DNA ends containing either a 5'-hydroxyl or 3'-phosphate requiring correction by the dual function enzyme, polynucleotide kinase phosphatase (PNKP), before DNA polymerase and ligation reactions can occur to seal the break. Pnkp gene deletion during early murine development leads to lethality; in contrast, the role of PNKP in adult mice is unknown. To investigate the latter, we used an inducible conditional mutagenesis approach to cause global disruption of the Pnkp gene in adult mice. This resulted in a premature aging-like phenotype, characterized by impaired growth of hair follicles, seminiferous tubules, and neural progenitor cell populations. These results point to an important role for PNKP in maintaining the normal growth and survival of these murine progenitor populations.<br /> (© 2021 Shin et al.)
- Subjects :
- Adult Stem Cells cytology
Adult Stem Cells metabolism
Animals
Apoptosis
Biomarkers
Cell Differentiation genetics
DNA Damage
DNA Repair
Dermis cytology
Dermis metabolism
Fluorescent Antibody Technique
Germ Cells cytology
Germ Cells metabolism
Hair Follicle cytology
Hair Follicle metabolism
Hyperpigmentation genetics
Immunohistochemistry
Melanins metabolism
Mice
Mice, Knockout
Cell Self Renewal genetics
Phosphotransferases (Alcohol Group Acceptor) genetics
Stem Cells cytology
Stem Cells metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2575-1077
- Volume :
- 4
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Life science alliance
- Publication Type :
- Academic Journal
- Accession number :
- 34226276
- Full Text :
- https://doi.org/10.26508/lsa.202000790