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Near-Infrared Small Molecule as a Specific Fluorescent Probe for Ultrasensitive Recognition of Antiparallel Human Telomere G-Quadruplexes.
- Source :
-
ACS applied materials & interfaces [ACS Appl Mater Interfaces] 2021 Jul 21; Vol. 13 (28), pp. 32743-32752. Date of Electronic Publication: 2021 Jul 06. - Publication Year :
- 2021
-
Abstract
- In the past 10 years, many fluorescent probes have been developed to recognize G-quadruplexes (G4s) since G4s play an important role in biological systems. However, the selectivity and sensitivity of existing probes for G4s limit their further applications. Herein, we design and synthesize a new probe (TOVJ) by introducing 9-vinyljulolidine into TO. The new probe exhibits almost no fluorescence in an aqueous solution. Upon interacting with G4s, especially the antiparallel G4s, the fluorescence intensity was greatly enhanced (maximum 2742-fold) with a large Stokes shift of 198 nm and the maximum emission peak at 694 nm (near-infrared region). TOVJ showed high sensitivity and selectivity to G4s over other DNA topologies (ssDNA/dsDNA), especially to antiparallel G4s. For antiparallel human telomere G4 detection, the limits of detection of Hum24 and 22AG Na <superscript>+</superscript> were as low as 164 and 231 pM, respectively. This indicates that TOVJ is a highly sensitive fluorescence sensor that can be effectively used for antiparallel human telomere G4 detection. The result of live-cell imaging showed that TOVJ could enter live cells and locate in the mitochondria.
- Subjects :
- DNA genetics
Fluorescent Dyes chemical synthesis
HeLa Cells
Humans
Limit of Detection
Microscopy, Fluorescence
Quinolines chemical synthesis
Quinolizines chemical synthesis
RNA analysis
RNA genetics
DNA analysis
Fluorescent Dyes chemistry
G-Quadruplexes
Quinolines chemistry
Quinolizines chemistry
Telomere chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1944-8252
- Volume :
- 13
- Issue :
- 28
- Database :
- MEDLINE
- Journal :
- ACS applied materials & interfaces
- Publication Type :
- Academic Journal
- Accession number :
- 34228441
- Full Text :
- https://doi.org/10.1021/acsami.1c07101