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Ectopic expression of an expansin-like B gene from wild Arachis enhances tolerance to both abiotic and biotic stresses.
- Source :
-
The Plant journal : for cell and molecular biology [Plant J] 2021 Sep; Vol. 107 (6), pp. 1681-1696. Date of Electronic Publication: 2021 Jul 28. - Publication Year :
- 2021
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Abstract
- Plant expansins are structural cell wall-loosening proteins implicated in several developmental processes and responses to environmental constraints and pathogen infection. To date, there is limited information about the biological function of expansins-like B (EXLBs), one of the smallest and less-studied subfamilies of plant expansins. In the present study, we conducted a functional analysis of the wild Arachis AdEXLB8 gene in transgenic tobacco (Nicotiana tabacum) plants to clarify its putative role in mediating defense responses to abiotic and biotic stresses. First, its cell wall localization was confirmed in plants expressing an AdEXLB8:eGFP fusion protein, while nanomechanical assays indicated cell wall reorganization and reassembly due to AdEXLB8 overexpression without compromising the phenotype. We further demonstrated that AdEXLB8 increased tolerance not only to isolated abiotic (drought) and biotic (Sclerotinia sclerotiorum and Meloidogyne incognita) stresses but also to their combination. The jasmonate and abscisic acid signaling pathways were clearly favored in transgenic plants, showing an activated antioxidative defense system. In addition to modifications in the biomechanical properties of the cell wall, we propose that AdEXLB8 overexpression interferes with phytohormone dynamics leading to a defense primed state, which culminates in plant defense responses against isolated and combined abiotic and biotic stresses.<br /> (© 2021 Society for Experimental Biology and John Wiley & Sons Ltd.)
- Subjects :
- Abscisic Acid metabolism
Animals
Ascomycota pathogenicity
Biomechanical Phenomena
Cell Wall genetics
Cell Wall metabolism
Cyclopentanes metabolism
Droughts
Gene Expression Regulation, Plant
Oxylipins metabolism
Plant Cells metabolism
Plant Leaves physiology
Plant Proteins metabolism
Plants, Genetically Modified
Recombinant Fusion Proteins genetics
Recombinant Fusion Proteins metabolism
Nicotiana cytology
Nicotiana genetics
Nicotiana microbiology
Tylenchoidea pathogenicity
Arachis genetics
Plant Proteins genetics
Stress, Physiological genetics
Nicotiana physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1365-313X
- Volume :
- 107
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- The Plant journal : for cell and molecular biology
- Publication Type :
- Academic Journal
- Accession number :
- 34231270
- Full Text :
- https://doi.org/10.1111/tpj.15409