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Spatiotemporal expression of Rap1 and Ras mediates the acquisition and reinstatement of methamphetamine-induced conditioned place preference in mice via extracellular signal-regulated kinase activation.
- Source :
-
Neuroreport [Neuroreport] 2021 Aug 11; Vol. 32 (12), pp. 1035-1040. - Publication Year :
- 2021
-
Abstract
- Drug addiction is a chronic recurrent brain disease characterized by compulsive drug use and a high tendency to relapse. We previously reported that the Ras-extracellular signal-regulated kinase (ERK)-ΔFosB pathway in the caudate putamen (CPu) was involved in methamphetamine-induced behavioral sensitization. Rap1, as an antagonist of Ras originally, was found to participate in neuronal synaptic plasticity recently, but the role of Rap1 in methamphetamine addiction is unclear. First, in this study, we constructed the acquisition, extinction and reinstatement of methamphetamine-induced conditioned place preference (CPP) in mice, respectively. Then, protein levels of Rap1, Ras and pERK/ERK in the prefrontal cortex (PFc), CPu and hippocampus of CPP mice on three phases were detected. We found that protein levels of Rap1, Ras and pERK/ERK in the CPu were significantly increased after repeated methamphetamine administration, as well as Rap1 and pERK/ERK in the hippocampus. However, protein levels of Rap1 and pERK/ERK in the CPu were decreased on the reinstatement of CPP mice. Therefore, Rap1 and Ras in the CPu and Rap1 in the hippocampus may participate in the regulation of the acquisition of methamphetamine-induced CPP in mice by activating ERK. Moreover, Rap1-ERK cascade in the CPu contributes to both the acquisition and reinstatement of methamphetamine-induced CPP in mice.<br /> (Copyright © 2021 Wolters Kluwer Health, Inc. All rights reserved.)
- Subjects :
- Animals
Conditioning, Operant physiology
Gene Expression
Hippocampus drug effects
Hippocampus metabolism
Male
Mice
Mice, Inbred C57BL
Prefrontal Cortex drug effects
Prefrontal Cortex metabolism
rap1 GTP-Binding Proteins genetics
ras Proteins genetics
Central Nervous System Stimulants pharmacology
Conditioning, Operant drug effects
Extracellular Signal-Regulated MAP Kinases metabolism
Methamphetamine pharmacology
rap1 GTP-Binding Proteins biosynthesis
ras Proteins biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 1473-558X
- Volume :
- 32
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Neuroreport
- Publication Type :
- Academic Journal
- Accession number :
- 34232127
- Full Text :
- https://doi.org/10.1097/WNR.0000000000001686