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A new flavor of cellular Atg8-family protein lipidation - alternative conjugation to phosphatidylserine during CASM.

Authors :
Durgan J
Florey O
Source :
Autophagy [Autophagy] 2021 Sep; Vol. 17 (9), pp. 2642-2644. Date of Electronic Publication: 2021 Jul 12.
Publication Year :
2021

Abstract

Atg8-family protein lipidation is the most commonly used marker for monitoring autophagy. During macroautophagy, Atg8-family proteins are specifically conjugated to phosphatidylethanolamine (PE) in forming, double-membrane autophagosomes. A distinct, non-canonical autophagy pathway also operates, characterized by the Conjugation of ATG8s to endolysosomal Single Membranes (CASM). In our new study, we show that CASM is associated with the alternative conjugation of Atg8-family proteins to phosphatidylserine (PS), and PE, in response to various cellular stimuli. We also discover differences in the regulation of conjugation to PE and PS by ATG4s, and altered dynamics between the two species. The identification of alternative Atg8-family protein PS lipidation opens up exciting new questions on the roles, regulation and biology of Atg8-family proteins during non-canonical autophagy.

Details

Language :
English
ISSN :
1554-8635
Volume :
17
Issue :
9
Database :
MEDLINE
Journal :
Autophagy
Publication Type :
Academic Journal
Accession number :
34251968
Full Text :
https://doi.org/10.1080/15548627.2021.1947730