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β-amyrin-induced apoptosis in Candida albicans triggered by calcium.
- Source :
-
Fungal biology [Fungal Biol] 2021 Aug; Vol. 125 (8), pp. 630-636. Date of Electronic Publication: 2021 Apr 04. - Publication Year :
- 2021
-
Abstract
- The emergence of drug-resistant pathogens has urged researchers to discover alternatives for traditional antibiotics. β-amyrin, which is included in the category of triterpenoids extracted from plants, is known for its antimicrobial activity, although the underlying mechanism has not yet been revealed. This study was conducted to elucidate the antifungal mode of action of β-amyrin against Candida albicans. Based on the relevance between triterpenoids and oxidative molecules, reactive oxygen species (ROS) concentrations were detected, which showed a noticeable increment. Disruption of Ca <superscript>2+</superscript> homeostasis in the cytosol was additionally analyzed, which was supported by interactions between two. Subsequently, decrease in mitochondrial membrane potential, increment of mitochondrial Ca <superscript>2+</superscript> , and ROS concentration were monitored, which suggested mitochondrial dysfunction modulated by Ca <superscript>2+</superscript> . Further investigation confirmed oxidative damage through glutathione reduction and DNA fragmentation. Accumulation of lethal damages resulted in the activation of caspases and externalization of phosphatidylserine, indicating the induction of yeast apoptosis by β-amyrin in C. albicans.<br />Competing Interests: Declaration of competing interest The authors declare that they have no conflict of interest.<br /> (Copyright © 2021 British Mycological Society. Published by Elsevier Ltd. All rights reserved.)
- Subjects :
- Antifungal Agents pharmacology
Membrane Potential, Mitochondrial drug effects
Mitochondria drug effects
Oleanolic Acid metabolism
Oleanolic Acid pharmacology
Reactive Oxygen Species analysis
Apoptosis drug effects
Calcium metabolism
Calcium pharmacology
Candida albicans drug effects
Oleanolic Acid analogs & derivatives
Subjects
Details
- Language :
- English
- ISSN :
- 1878-6146
- Volume :
- 125
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Fungal biology
- Publication Type :
- Academic Journal
- Accession number :
- 34281656
- Full Text :
- https://doi.org/10.1016/j.funbio.2021.03.006